一种具有κ轻链大细胞质内含物的小鼠杂交瘤。
A murine hybridoma with large cytoplasmic inclusions of kappa light chains.
作者信息
Reason D C
出版信息
J Exp Med. 1987 Feb 1;165(2):578-83. doi: 10.1084/jem.165.2.578.
Abstract
A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein.
摘要
已建立了一种鼠杂交瘤细胞系，该细胞系始终会形成大的细胞质内含物。当进行原位染色时，这些结构会结合针对小鼠κ轻链的特异性抗体。对分离出的包涵体进行SDS-PAGE分析，产生一条约26,000 Mr的单一蛋白带，转移至硝酸纤维素膜后与抗κ抗体发生反应。在纯化的蛋白质中未检测到碳水化合物。这些数据与完整κ轻链蛋白的细胞内滞留和沉积一致。
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