Department of Civil and Environmental Engineering, CIE 201, NDSU, Fargo, ND 58104, United States of America.
Department of Civil and Environmental Engineering, CIE 201, NDSU, Fargo, ND 58104, United States of America.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Feb 5;208:85-96. doi: 10.1016/j.saa.2018.09.052. Epub 2018 Oct 1.
Breast cancer is a global health issue and the second leading cause of cancer death in women. Breast cancer tends to migrate to bone and causes bone metastases which is ultimately the cause of death. Here, we report the use of FTIR to identify spectral biomarkers of cancer progression on 3D in vitro model of breast cancer bone metastasis. Our results indicate that the following spectral biomarkers can monitor cancer progression, for example, lipids (CH2 asymmetric/CH2 symmetric stretch), Amide I/Amide II, and RNA/DNA. Principal component analysis also confirmed the involvement of protein, lipids and nucleic acids in cancer progression on sequential culture. The collective observations from this study suggest successful application of FTIR as a non-invasive and accurate method to identify biochemical changes in cancer cells during the progression of breast cancer bone metastasis.
乳腺癌是一个全球性的健康问题，也是女性癌症死亡的第二大主要原因。乳腺癌往往会转移到骨骼并引起骨转移，这最终是导致死亡的原因。在这里，我们报告了使用傅里叶变换红外光谱（FTIR）来识别乳腺癌骨转移的 3D 体外模型中癌症进展的光谱生物标志物。我们的结果表明，以下光谱生物标志物可用于监测癌症进展，例如脂质（CH2 不对称/CH2 对称伸缩）、酰胺 I/酰胺 II 和 RNA/DNA。主成分分析也证实了在连续培养过程中蛋白质、脂质和核酸参与了癌症的进展。这项研究的综合观察结果表明，FTIR 作为一种非侵入性和准确的方法，可以成功应用于识别乳腺癌骨转移过程中癌细胞的生化变化。
