Ravi Preetham, Jasuja Haneesh, Sarkar Dipayan, Vahidi Pashaki Benjamin, Gaikwad Hanmant K, Vahidi Pashaki Pooyan, Katti Dinesh R, Shetty Kalidas, Katti Kalpana S
Department of Civil, Construction and Environmental Engineering, North Dakota State University, 1410 14th Ave N, Fargo, ND, 58105, USA.
United States Department of Agriculture, Fargo, ND, 58105, USA.
Sci Rep. 2025 Mar 18;15(1):9341. doi: 10.1038/s41598-025-93274-0.
Breast cancer cells have the propensity to metastasize to bone, resulting in altered growth, chemoresistance, and causing skeletal failures, often leading to death in patients. There is a scarcity of effective therapeutics for bone metastasized breast cancer due to the lack of accurate drug screening metastasis models. We utilize a unique 3D in vitro nano clay-based scaffold model as a testbed for bone metastatic breast cancer for drug screening applications. Rhodiola crenulata, a Tibetan plant-based extract, has been previously explored for primary-site breast cancer. However, its effect on bone metastasized breast cancer cells is unknown. In the present study, we evaluated the cytotoxicity of R. crenulata extract on bone metastatic breast cancer using testbeds and compared the results with 2D cultured cells. We observed that R. crenulata induced apoptosis in bone metastasized breast cancer cells grown on a 3D in vitro testbed by upregulating pro-apoptotic proteins, p53, and caspase-9. Alternatively, we observed that bone cells remain unaffected by the treatment of R. crenulata. For the first time, we demonstrated the anticancer capabilities of R. crenulata against bone metastasis of breast cancer. R. crenulata is a robust therapeutic candidate for bone metastasis, shown to induce death in bone metastatic breast cancer while unaffecting healthy bone.
乳腺癌细胞有向骨转移的倾向，会导致生长改变、产生化学抗性，并引发骨骼衰竭，常常导致患者死亡。由于缺乏准确的药物筛选转移模型，针对骨转移乳腺癌的有效治疗方法匮乏。我们利用一种独特的基于纳米粘土的三维体外支架模型作为骨转移性乳腺癌药物筛选应用的试验平台。红景天，一种基于藏药植物的提取物，此前已被研究用于原发性乳腺癌。然而，其对骨转移乳腺癌细胞的作用尚不清楚。在本研究中，我们使用试验平台评估了红景天提取物对骨转移乳腺癌的细胞毒性，并将结果与二维培养细胞进行了比较。我们观察到，红景天通过上调促凋亡蛋白p53和半胱天冬酶-9，诱导在三维体外试验平台上生长的骨转移乳腺癌细胞发生凋亡。另外，我们观察到骨细胞不受红景天处理的影响。我们首次证明了红景天对乳腺癌骨转移具有抗癌能力。红景天是一种用于治疗骨转移的有力候选药物，它能在不影响健康骨骼的情况下，诱导骨转移乳腺癌细胞死亡。
