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红外显微光谱和成像分析炎症性和非炎症性乳腺癌细胞及其糖胺聚糖分泌组。

Infrared Microspectroscopy and Imaging Analysis of Inflammatory and Non-Inflammatory Breast Cancer Cells and Their GAG Secretome.

机构信息

Laboratoire de Biochimie Médicale et Biologie Moléculaire, Université de Reims Champagne-Ardenne, 51097 Reims, France.

CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire-MEDyC, 51097 Reims, France.

出版信息

Molecules. 2020 Sep 19;25(18):4300. doi: 10.3390/molecules25184300.

DOI:10.3390/molecules25184300
PMID:32961706
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7570935/
Abstract

Glycosaminoglycans (GAGs)/proteoglycans (PGs) play a pivotal role in the metastasis of inflammatory breast cancer (IBC). They represent biomarkers and targets in diagnosis and treatment of different cancers including breast cancer. Thus, GAGs/PGs could represent potential prognostic/diagnostic biomarkers for IBC. In the present study, non-IBC MDA-MB-231, MCF7, SKBR3 cells and IBC SUM149 cells, as well as their GAG secretome were analyzed. The latter was measured in toto as dried drops with high-throughput (HT) Fourier Transform InfraRed (FTIR) spectroscopy and imaging. FTIR imaging was also employed to investigate single whole breast cancer cells while synchrotron-FTIR microspectroscopy was used to specifically target their cytoplasms. Data were analyzed by hierarchical cluster analysis and principal components analysis. Results obtained from HT-FTIR analysis of GAG drops showed that the inter-group variability enabled us to delineate between cell types in the GAG absorption range 1350-800 cm. Similar results were obtained for FTIR imaging of GAG extracts and fixed single whole cells. Synchrotron-FTIR data from cytoplasms allowed discrimination between non-IBC and IBC. Thus, by using GAG specific region, not only different breast cancer cell lines could be differentiated, but also non-IBC from IBC cells. This could be a potential diagnostic spectral marker for IBC detection useful for patient management.

摘要

糖胺聚糖（GAGs）/蛋白聚糖（PGs）在炎症性乳腺癌（IBC）的转移中起着关键作用。它们是不同癌症（包括乳腺癌）诊断和治疗的生物标志物和靶点。因此，GAGs/PGs 可能是 IBC 的潜在预后/诊断生物标志物。在本研究中，分析了非 IBC MDA-MB-231、MCF7、SKBR3 细胞和 IBC SUM149 细胞及其 GAG 分泌组。后者通过高通量（HT）傅里叶变换红外（FTIR）光谱和成像以干燥液滴的形式进行了全面测量。FTIR 成像还用于研究单个全乳腺癌细胞，而同步辐射-FTIR 微光谱则用于专门针对其细胞质。通过层次聚类分析和主成分分析对数据进行了分析。通过对 GAG 液滴的 HT-FTIR 分析获得的数据表明，组间变异性使我们能够在 GAG 吸收范围 1350-800 cm 内区分细胞类型。GAG 提取物和固定的单个全细胞的 FTIR 成像也获得了类似的结果。来自细胞质的同步辐射-FTIR 数据允许区分非 IBC 和 IBC。因此，通过使用 GAG 特定区域，不仅可以区分不同的乳腺癌细胞系，还可以区分非 IBC 和 IBC 细胞。这可能是一种潜在的用于 IBC 检测的诊断光谱标记物，有助于患者管理。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffff/7570935/960642eb94d4/molecules-25-04300-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffff/7570935/506cc49a7194/molecules-25-04300-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffff/7570935/52a3ee74466e/molecules-25-04300-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffff/7570935/4b2327ff4573/molecules-25-04300-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffff/7570935/960642eb94d4/molecules-25-04300-g007.jpg

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