Composting Research group, Department of Chemical, Biological and Environmental Engineering. Escola d'Enginyeria, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, 08193, Barcelona, Spain.
Appl Microbiol Biotechnol. 2018 Dec;102(23):9991-10004. doi: 10.1007/s00253-018-9384-8. Epub 2018 Oct 6.
2-Phenylethanol (2-PE) and 2-phenethyl acetate (2-PEA) are valuable generally recognized as safe flavoring agents widely used in industry. Perfumes, pharmaceuticals, polishes, and personal care products, are some of the final products using these compounds as additives due to their rose-like odor. Also, 2-PE is used in disinfectants, pest control, and cleaning products due to its biocide capability. Although most of these additives production are derived from chemical synthesis, the current trend of consumers to prefer natural products has contributed to the development of biotechnological approaches as an alternative way to obtain natural 2-PE and 2-PEA. The most efficient route to bioproduce these compounds is through the bioconversion of L-phenylalanine via the Ehrlich pathway, and most of the advances have been focused on the development of this process. This review compiles the most recent developments in the biotechnological production of 2-PE and 2-PEA, indicating the most studied strains producing 2-PE and 2-PEA, the current advances in the in situ product recovery in liquid systems, an overview of the strain developments, and the progress in the use of residue-based systems. Future research should address the need for more sustainable and economic systems such as those using wastes as raw materials, as well as the scale-up of the proposed technologies.
2-苯乙醇（2-PE）和 2-苯乙醚（2-PEA）是两种有价值的、被广泛认可的安全香料，在工业中被广泛使用。香水、药品、抛光剂和个人护理产品是一些最终产品，这些化合物作为添加剂使用，因为它们具有玫瑰般的气味。此外，由于具有杀菌能力，2-PE 还被用于消毒剂、害虫防治和清洁产品。尽管这些添加剂的大部分生产都来自化学合成，但消费者对天然产品的偏好趋势促使生物技术方法得到发展，成为获得天然 2-PE 和 2-PEA 的替代方法。通过 Ehrlich 途径生物转化 L-苯丙氨酸是生物生产这些化合物最有效的途径，大多数进展都集中在这一过程的开发上。本文综述了生物法生产 2-PE 和 2-PEA 的最新进展，指出了研究最多的生产 2-PE 和 2-PEA 的菌株、液体体系中当前的原位产物回收进展、菌株开发概述以及基于残渣体系的应用进展。未来的研究应解决对更可持续和经济的系统的需求，例如使用废物作为原料，以及扩大拟议技术的规模。
