Bernardino Ana R S, Grosso Filipa, Torres Cristiana A V, Reis Maria A M, Peixe Luísa
UCIBIO - Applied Molecular Biosciences Unit, Department of Chemistry, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal.
Laboratory i4HB - Institute for Health and Bioeconomy, School of Science and Technology, NOVA University Lisbon, Caparica, Portugal.
Biotechnol Rep (Amst). 2024 Apr 4;42:e00839. doi: 10.1016/j.btre.2024.e00839. eCollection 2024 Jun.
A bacterium, ANG344B, isolated from river water, exhibited an exceptional capacity to produce 2-phenylethanol (2-PE) using L-phenylalanine (L-Phe) as a precursor-a capability typically observed in yeasts rather than bacteria. Bioreactor experiments were conducted to evaluate the production performance, using glucose as the carbon source for cellular growth and L-Phe as the precursor for 2-PE production. Remarkably, ANG344B achieved a 2-PE concentration of 2.35 ± 0.26 g/L in just 24.5 h of cultivation, exhibiting a global volumetric productivity of 0.10 ± 0.01 g/L.h and a production yield of 0.51 ± 0.01 g/g, a result hitherto reported only for yeasts. These findings position ANG344B as a highly promising microorganism for 2-PE production. Whole-genome sequencing of strain ANG344 revealed a genome size of 3.52 Mb with a GC content of 42.7 %. Utilizing the Rapid Annotation using Subsystem Technology (RAST) server, 3418 coding genes were predicted, including genes coding for enzymes previously associated with the metabolic pathway of 2-PE production in other microorganisms, yet unreported in species. Through gene mapping, 299 subsystems were identified, exhibiting 30 % subsystem coverage. The whole genome sequence data was submitted to NCBI GeneBank with the BioProject ID PRJNA982713. These draft genome data offer significant potential for exploiting the biotechnological capabilities of strain ANG344 and for conducting further comparative genomic studies.
从河水中分离出的一种细菌ANG344B，具有以L-苯丙氨酸（L-Phe）为前体生产2-苯乙醇（2-PE）的卓越能力，这种能力通常在酵母而非细菌中观察到。进行了生物反应器实验以评估生产性能，使用葡萄糖作为细胞生长的碳源，L-Phe作为2-PE生产的前体。值得注意的是，ANG344B在仅24.5小时的培养中就达到了2.35±0.26 g/L的2-PE浓度，显示出0.10±0.01 g/L·h的总体积生产力和0.51±0.01 g/g的产率，这一结果迄今为止仅在酵母中报道过。这些发现使ANG344B成为生产2-PE极具前景的微生物。ANG344菌株的全基因组测序显示基因组大小为3.52 Mb，GC含量为42.7%。利用基于子系统技术的快速注释（RAST）服务器，预测了3418个编码基因，包括编码与其他微生物中2-PE生产代谢途径相关的酶的基因，但在该物种中尚未报道。通过基因图谱分析，确定了299个子系统，子系统覆盖率为30%。全基因组序列数据已提交至NCBI基因库，生物项目编号为PRJNA982713。这些基因组草图数据为开发ANG344菌株的生物技术能力以及进行进一步的比较基因组研究提供了巨大潜力。
