[Experimental study on dendritic cells modified by dust mite allergen gene in the treatment of allergic rhinitis in mouse].

To explore the therapeutic effects of dendritic cells (DC) modified by the dust-mite-allergen(1) (). DC modified by the 1 gene (Der p1-DC) were prepared.Using random number table, 24 Balb\c mice were divided into four groups: immature DC (imDC)/AR group, dexamethasone/AR group, Der p1-DC/AR group and control group, with 6 mice in each group.AR mouse model was built with Der p1 and the mouse model of AR was established.The AR mice were respectively given by abdominal injection of Der p1-DC, imDC and dexamethasone.Normal control mice were treated with physiologic saline.ELISA method was used for determining the content of IgE, IgG1and histamine in blood.The relative expression of mRNA of IL-4 and IL-13 on nasal mucosa with protein was analyzed by RT-PCR and Westen blot methods.All the data were statistically analyzed by SPSS 19.0 statistical software, and the variance analysis was used in multiple groups of average samples. The contents of IgE, IgG1 and histamine in the mice of Der p1-DC/AR group were lower than those in imDC/AR group ((0.560±0.110) OD 450 nm (1.150±0.280) OD 450 nm, (0.690±0.054) OD 450 nm (0.920±0.125) OD 450 nm, (4 145±670) pg/ml (7 685±669) pg/ml, value was 4.80, 4.14, 9.16, respectively, all <0.05), and the expression of IL-4 and IL-13 on nasal mucosa in Der p1-DC/AR group was remarkedly lower than those in imDC/AR group (0.41±0.25 1.59±1.02, 0.26±0.01 1.10±0.09, value was 2.75, 22.72, respectively, all <0.05). There was no statistically significant difference between the mice treated with Der p1-DC and dexamethasone group. The results showed that Der p1-DC could reduce inflammation in AR mice and decrease the expression of IL-4 and IL-13. It suggested that Der p1-DC can be used in the immunotherapy of AR mouse.