Che N, Han B, Liu S X, Ge R M, Yu S Q
Department of Otorhinolaryngology Head and Neck Surgery, Tongji Hospital of Tongji University, Shanghai 200065, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2018 Sep 7;53(9):675-679. doi: 10.3760/cma.j.issn.1673-0860.2018.09.007.
To explore the therapeutic effects of dendritic cells (DC) modified by the dust-mite-allergen(1) (). DC modified by the 1 gene (Der p1-DC) were prepared.Using random number table, 24 Balb\c mice were divided into four groups: immature DC (imDC)/AR group, dexamethasone/AR group, Der p1-DC/AR group and control group, with 6 mice in each group.AR mouse model was built with Der p1 and the mouse model of AR was established.The AR mice were respectively given by abdominal injection of Der p1-DC, imDC and dexamethasone.Normal control mice were treated with physiologic saline.ELISA method was used for determining the content of IgE, IgG1and histamine in blood.The relative expression of mRNA of IL-4 and IL-13 on nasal mucosa with protein was analyzed by RT-PCR and Westen blot methods.All the data were statistically analyzed by SPSS 19.0 statistical software, and the variance analysis was used in multiple groups of average samples. The contents of IgE, IgG1 and histamine in the mice of Der p1-DC/AR group were lower than those in imDC/AR group ((0.560±0.110) OD 450 nm (1.150±0.280) OD 450 nm, (0.690±0.054) OD 450 nm (0.920±0.125) OD 450 nm, (4 145±670) pg/ml (7 685±669) pg/ml, value was 4.80, 4.14, 9.16, respectively, all <0.05), and the expression of IL-4 and IL-13 on nasal mucosa in Der p1-DC/AR group was remarkedly lower than those in imDC/AR group (0.41±0.25 1.59±1.02, 0.26±0.01 1.10±0.09, value was 2.75, 22.72, respectively, all <0.05). There was no statistically significant difference between the mice treated with Der p1-DC and dexamethasone group. The results showed that Der p1-DC could reduce inflammation in AR mice and decrease the expression of IL-4 and IL-13. It suggested that Der p1-DC can be used in the immunotherapy of AR mouse.
探讨经尘螨变应原（1）修饰的树突状细胞（DC）的治疗效果。制备经1基因修饰的DC（Der p1-DC）。采用随机数字表法，将24只Balb/c小鼠分为四组：未成熟DC（imDC）/AR组、地塞米松/AR组、Der p1-DC/AR组和对照组，每组6只。用Der p1建立AR小鼠模型并确立AR小鼠模型。对AR小鼠分别腹腔注射Der p1-DC、imDC和地塞米松。正常对照小鼠用生理盐水处理。采用ELISA法测定血液中IgE、IgG1和组胺的含量。采用RT-PCR和蛋白质免疫印迹法分析鼻黏膜上IL-4和IL-13 mRNA的相对表达。所有数据采用SPSS 19.0统计软件进行统计学分析，多组均数样本采用方差分析。Der p1-DC/AR组小鼠的IgE、IgG1和组胺含量低于imDC/AR组（（0.560±0.110）OD 450 nm比（1.150±0.280）OD 450 nm，（0.690±0.054）OD 450 nm比（0.920±0.125）OD 450 nm，（4145±670）pg/ml比（7685±669）pg/ml，P值分别为4.80、4.14、9.16，均<0.05），Der p1-DC/AR组鼻黏膜上IL-4和IL-13的表达明显低于imDC/AR组（0.41±0.25比1.59±1.02，0.26±0.01比1.10±0.09，P值分别为2.75、22.72，均<0.05）。Der p1-DC处理组与地塞米松组小鼠之间无统计学差异。结果表明，Der p1-DC可减轻AR小鼠的炎症并降低IL-4和IL-13的表达。提示Der p1-DC可用于AR小鼠的免疫治疗。
