Chipiti Talent, Ibrahim Mohammed Auwal, Singh Moganavelli, Islam Md Shahidul
Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4000, South Africa.
Pharmacogn Mag. 2017 Jul;13(Suppl 2):S329-S333. doi: 10.4103/pm.pm_223_16. Epub 2017 Jul 11.
Hyperglycemia is the hallmark of type 2 diabetes mellitus, and its prevention will go a long way in managing the disease and its associated complications. Reduction of postprandial hyperglycemia through retarding carbohydrates digesting enzymes is one of the major therapeutic approaches used in the management of diabetes.
The aim of the present study was to investigate the antidiabetic and cytotoxic effects of extracts .
The α-amylase and α-glucosidase inhibitory activities of ethanolic and aqueous extracts of root and leaves were investigated when the cytotoxic effects of these extracts were analyzed using MTT assay on human embryonic kidney (HEK 293) cell lines.
The root ethanolic extract showed a mild α-amylase inhibitory activity with IC value of 22.75 ± 1.23 μg/ml, but strong α-glucosidase inhibitory activity with IC value 2.81 ± 0.97 μg/ml and the aqueous root extract indicated moderate inhibition for both α-amylase and α-glucosidase with IC values of 33.70 ± 3.75 and 37.48 ± 2.35 μg/ml, respectively. The ethanolic root extract was found nontoxic at tested concentrations on HEK 293 cell lines as confirmed by the MTT assay with 93% cell viability at the highest concentration (200 μg/ml) tested. However, the aqueous extracts (leaf and root) were found cytotoxic at concentrations above 50 μg/ml.
Data of this study suggest that the root ethanolic extracts of possesses moderate α-amylase, but strong α-glucosidase inhibitory activity and did not show significant cytotoxicity with the tested concentrations.
Present study was conducted to examine effects of antidiabetic and cyctotoxic effects of Cissus conrnifolia root and leaves extracts . Data of this study suggest that the root ethanolic extract of possesses mild to moderated antidiabetic activity via inhibiting carbohydrate digesting enzymes when no significant toxicity was observed with tested concentrations. alex: Aqueous leaf extract; arex: Aqueous root extract; CC: ; DNS: Dinitrosalicylic acid; DMSO: Dimethylsulfoxide; elex: Ethanolic leaf extract; erex: Ethanolic root extract; IDF: International Diabetes Federation; MEM: Minimum essential medium; NIDDM: Noninsulin-dependent diabetes mellitus; pNPG: Para-nitrophenyl glucopyranoside; SD: Standard deviation; T2D: Type 2 diabetes.
高血糖是2型糖尿病的标志，预防高血糖对控制该疾病及其相关并发症具有重要意义。通过抑制碳水化合物消化酶来降低餐后高血糖是糖尿病治疗的主要方法之一。
本研究旨在探讨[植物名称]提取物的抗糖尿病和细胞毒性作用。
研究了[植物名称]根和叶的乙醇提取物及水提取物对α-淀粉酶和α-葡萄糖苷酶的抑制活性，并采用MTT法分析这些提取物对人胚肾（HEK 293）细胞系的细胞毒性作用。
根乙醇提取物对α-淀粉酶表现出轻度抑制活性，IC50值为22.75±1.23μg/ml，但对α-葡萄糖苷酶具有较强的抑制活性，IC50值为2.81±0.97μg/ml；根水提取物对α-淀粉酶和α-葡萄糖苷酶均表现出中度抑制作用，IC50值分别为33.70±3.75和37.48±2.35μg/ml。MTT法检测结果证实，根乙醇提取物在测试浓度下对HEK 293细胞系无毒，在最高测试浓度（200μg/ml）下细胞活力为93%。然而，水提取物（叶和根）在浓度高于50μg/ml时具有细胞毒性。
本研究数据表明，[植物名称]根乙醇提取物具有中度的α-淀粉酶抑制活性，但α-葡萄糖苷酶抑制活性较强，且在测试浓度下未显示出明显的细胞毒性。
本研究旨在考察[植物名称]根和叶提取物的抗糖尿病和细胞毒性作用。本研究数据表明，[植物名称]根乙醇提取物通过抑制碳水化合物消化酶具有轻度至中度的抗糖尿病活性，且在测试浓度下未观察到明显毒性。 alex：叶水提取物；arex：根水提取物；CC：[植物名称]；DNS：二硝基水杨酸；DMSO：二甲基亚砜；elex：叶乙醇提取物；erex：根乙醇提取物；IDF：国际糖尿病联合会；MEM：最低限度基本培养基；NIDDM：非胰岛素依赖型糖尿病；pNPG：对硝基苯基吡喃葡萄糖苷；SD：标准差；T2D：2型糖尿病
