a College of Materials Science and Engineering , Zhejiang University of Technology , Hangzhou , China.
b Department of Otorhinolaryngology, The Second Affiliated Hospital, School of Medicine , Zhejiang University , Hangzhou , China.
J Biomater Sci Polym Ed. 2018 Dec;29(17):2154-2167. doi: 10.1080/09205063.2018.1528519. Epub 2018 Nov 24.
The wound healing for tympanic membrane (TM) perforations is one of the most common clinical treatment in otomicrosurgery. Recently, tissue-engineered composites and grafts, also new designs of biomaterials are applied to the management of TM perforation. In this work, a series of gelatin/genipin nanofibrous films were prepared as a patch for repairing TM perforation. Gelatin, a type of biomaterial with excellent electrospinning performance, has been used for preparing the nanofibers. The genipin, as a crosslinking agent, has been blended into the gelatin nanofibers. The reaction between gelatin and genipin engender suitable tensile strength and water-resistance for TM patch. The survival rate of human umbilical vein endothelial cells and fibroblasts demonstrated that the gelatin/genipin nanofiber scaffolds had good biocompatibility, which indicated the genipin was a kind of effective and nontoxic crosslinking agent for improving the mechanical property and water-resistance of gelatin films. In short, our work provides a novel macromolecular material with good mechanical properties, water-tolerance and excellent biocompatibility which could be used as a potential patch for TM repair.
鼓膜（TM）穿孔的伤口愈合是耳显微外科学中最常见的临床治疗方法之一。最近，组织工程复合材料和移植物，以及生物材料的新设计，已被应用于 TM 穿孔的管理。在这项工作中，一系列明胶/京尼平纳米纤维膜被制备为修复 TM 穿孔的补丁。明胶是一种具有优异的静电纺丝性能的生物材料，已被用于制备纳米纤维。京尼平作为一种交联剂，已被掺入明胶纳米纤维中。明胶和京尼平之间的反应产生了适合 TM 补丁的拉伸强度和耐水性。人脐静脉内皮细胞和成纤维细胞的存活率表明，明胶/京尼平纳米纤维支架具有良好的生物相容性，这表明京尼平是一种有效且无毒的交联剂，可提高明胶膜的机械性能和耐水性。总之，我们的工作提供了一种具有良好机械性能、耐水性和优异生物相容性的新型高分子材料，可作为 TM 修复的潜在补丁。
