Material Science and Engineering, College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou 310000, China.
The Department of Otolaryngology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China.
Molecules. 2022 May 3;27(9):2906. doi: 10.3390/molecules27092906.
Tympanic membrane perforation (TMP), a common disease, often needs a scaffold as the patch to support surgery. Due to the environment of auditory meatus, the patch can be infected by bacteria that results in failure; therefore, the ideal scaffold may combine biomimetic and antibacterial features. In this work, gelatin was used as the electrospinning framework, genipin as the crosslinking agent, and levofloxacin as an antibacterial in order to prepare the scaffold for TMP. Different contents of levofloxacin have been added to gelatin/genipin. It was found that, with the addition of levofloxacin, the gelatin/genipin membranes exhibit improved hydrophilia and enhanced tensile strength. The antibacterial and cell-cultured experiments showed that the prepared antibacterial membranes had excellent antibacterial properties and good biocompatibility, respectively. In summary, levofloxacin is a good group for the gelatin/genipin scaffold because it improves the physical properties and antibacterial action. Compared with different amounts of levofloxacin, a gelatin/genipin membrane with 1% levofloxacin is more suitable for a TM.
鼓膜穿孔（TMP）是一种常见疾病，通常需要支架作为贴片来支持手术。由于耳道口的环境，贴片可能会被细菌感染导致手术失败；因此，理想的支架可能结合仿生和抗菌特性。在这项工作中，明胶被用作静电纺丝的支架，京尼平作为交联剂，左氧氟沙星作为抗菌剂，以制备 TMP 的支架。向明胶/京尼平中添加了不同含量的左氧氟沙星。结果发现，随着左氧氟沙星的加入，明胶/京尼平膜的亲水性得到改善，拉伸强度得到提高。抗菌和细胞培养实验表明，所制备的抗菌膜具有优异的抗菌性能和良好的生物相容性。综上所述，左氧氟沙星是明胶/京尼平支架的一个很好的基团，因为它提高了物理性能和抗菌作用。与不同含量的左氧氟沙星相比，含有 1%左氧氟沙星的明胶/京尼平膜更适合 TM。
