Pham Minh Ngoc, Schuweiler Mark, Ismat Afshan
Department of Biology, Franklin & Marshall College, Lancaster, Pennsylvania.
Department of Biology, University of St. Thomas, Saint Paul, Minnesota.
Genesis. 2018 Dec;56(11-12):e23255. doi: 10.1002/dvg.23255. Epub 2018 Nov 2.
Vein patterning in the Drosophila wing provides a powerful tool to study regulation of various signaling pathways. Here we show that the ADAMTS extracellular protease AdamTS-B (CG4096) is expressed in the embryonic wing imaginal disc precursor cells and the wing imaginal disc, and functions to inhibit wing vein formation. Knock-down of AdamTS-B displayed posterior crossveins (PCVs) with either extra branches or deltas, or wider PCVs, and a wandering distal tip of the L5 longitudinal vein. Conversely, over-expression of AdamTS-B resulted in a complete absence of the PCV, an incomplete anterior crossvein, and missing distal end of the L5 longitudinal vein. We conclude that AdamTS-B inhibits wing vein formation through negative regulation of signaling pathways, possibly BMP as well as Egfr, displaying the complexity of roles for this family of extracellular proteases.
果蝇翅膀中的静脉模式为研究各种信号通路的调控提供了一个强大的工具。我们在此表明，ADAMTS细胞外蛋白酶AdamTS-B（CG4096）在胚胎翅膀成虫盘前体细胞和翅膀成虫盘中表达，并起到抑制翅膀静脉形成的作用。敲低AdamTS-B会显示出带有额外分支或三角的后交叉静脉（PCV），或者更宽的PCV，以及L5纵静脉的游走远端尖端。相反，AdamTS-B的过表达导致PCV完全缺失、前交叉静脉不完全以及L5纵静脉远端缺失。我们得出结论，AdamTS-B通过对信号通路的负调控来抑制翅膀静脉形成，可能是BMP以及Egfr，这显示了该细胞外蛋白酶家族作用的复杂性。
