Biological Sciences Institute, Federal University of Pará, Belém, Pará, Brazil.
Technology Institute, Federal University of Pará, Belém, Pará, Brazil.
Toxicol Appl Pharmacol. 2018 Dec 1;360:193-200. doi: 10.1016/j.taap.2018.10.008. Epub 2018 Oct 5.
This study aimed at describing the characteristics and properties of seizures induced by cunaniol, a polyacetylenic alcohol isolated from the Clibadium genus, which is ubiquitous in the Amazon biodiversity and its potential use as a convulsant model. Wistar rat behavior was assessed upon cunaniol administration and animals were evaluated for neural activity through electroencephalographic records whereby epidural electrodes were positioned over the motor cortex under cunaniol-elicited seizures and seizure's control using three anticonvulsant agents, namely phenytoin, phenobarbital and diazepam. Cunaniol-induced seizures displayed a cyclic development of electrocorticographic seizures, presenting interictal-like spike and ictal period, which correlates to the behavioral observations and is in line with acute seizures induced by pentylenetetrazole. Cunaniol-elicited seizures were intractable by phenytoin treatment and controlled under the GABAergic activities of phenobarbital and diazepam. The results indicate that the cunaniol-induced changes show characteristics of seizure activity, making this plant compound a suitable animal convulsant model for seizure-related studies that could be used to assist in the development of novel anticonvulsant agents.
本研究旨在描述从亚马逊生物多样性中普遍存在的 Clibadium 属中分离出的多炔醇 cunaniol 诱发的癫痫发作的特征和性质，以及其作为致惊厥模型的潜在用途。在给予 cunaniol 后评估 Wistar 大鼠的行为，并通过脑电图记录评估动物的神经活动，其中在 cunaniol 诱发的癫痫发作和癫痫发作的控制下，在运动皮层上放置硬膜外电极，使用三种抗惊厥剂苯妥英、苯巴比妥和地西泮。Cunaniol 诱导的癫痫发作呈现出脑电图癫痫发作的周期性发展，表现出发作间期样棘波和发作期，这与行为观察结果一致，与戊四氮诱导的急性癫痫发作一致。苯妥英治疗不能控制 cunaniol 诱发的癫痫发作，而苯巴比妥和地西泮的 GABA 能活性可控制其发作。结果表明，cunaniol 诱导的变化表现出癫痫活动的特征，使这种植物化合物成为与癫痫相关研究的合适动物惊厥模型，可用于辅助新型抗惊厥药物的开发。
