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大肠杆菌rep基因：基因序列、编码的解旋酶及其与uvrD的同源性。

Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD.

作者信息

Gilchrist C A, Denhardt D T

出版信息

Nucleic Acids Res. 1987 Jan 26;15(2):465-75. doi: 10.1093/nar/15.2.465.

DOI:10.1093/nar/15.2.465
PMID:3029683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340446/
Abstract

The sequence of a 2.67-kilobase section of the Escherichia coli chromosome that contains the rep gene has been determined. This gene codes for a protein of predicted Mr 72,800, a DNA helicase, which is also a single-stranded DNA-dependent ATPase. The sequenced region contains an open reading frame of the correct length and orientation to encode the Rep protein. A secondary structure for the protein can be formulated from the amino acid sequence. We have compared both the primary and the secondary structures of Rep with other proteins and find the greatest homology between Rep and E. coli helicase II, the product of the uvrD gene.

摘要

已确定大肠杆菌染色体中包含rep基因的一段2.67千碱基片段的序列。该基因编码一种预测分子量为72,800的蛋白质，即一种DNA解旋酶，它也是一种依赖单链DNA的ATP酶。测序区域包含一个长度和方向正确的开放阅读框，可编码Rep蛋白。可以根据氨基酸序列构建该蛋白质的二级结构。我们已将Rep的一级和二级结构与其他蛋白质进行了比较，发现Rep与uvrD基因产物大肠杆菌解旋酶II之间的同源性最高。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec53/340446/dcbf30fe329f/nar00246-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec53/340446/bf24908d1c97/nar00246-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec53/340446/dcbf30fe329f/nar00246-0095-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec53/340446/bf24908d1c97/nar00246-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ec53/340446/dcbf30fe329f/nar00246-0095-b.jpg

相似文献

1
Escherichia coli rep gene: sequence of the gene, the encoded helicase, and its homology with uvrD.大肠杆菌rep基因：基因序列、编码的解旋酶及其与uvrD的同源性。
Nucleic Acids Res. 1987 Jan 26;15(2):465-75. doi: 10.1093/nar/15.2.465.
2
The molecular cloning of the gene encoding the Escherichia coli 75-kDa helicase and the determination of its nucleotide sequence and gentic map position.编码大肠杆菌75-kDa解旋酶的基因的分子克隆及其核苷酸序列和遗传图谱位置的确定。
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The E. coli uvrD gene product is DNA helicase II.大肠杆菌uvrD基因产物是DNA解旋酶II。
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引用本文的文献

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E. coli Rep helicase and RecA recombinase unwind G4 DNA and are important for resistance to G4-stabilizing ligands.大肠杆菌 Rep 解旋酶和 RecA 重组酶可解开 G4 DNA，并且对于抵抗 G4 稳定配体很重要。
Nucleic Acids Res. 2020 Jul 9;48(12):6640-6653. doi: 10.1093/nar/gkaa442.
2
DNA Helicases.DNA解旋酶
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PriA participates in nascent DNA synthesis in Escherichia coli.PriA 参与大肠杆菌中新合成 DNA 的合成。

本文引用的文献

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Synthetic sites for transcription termination and a functional comparison with tryptophan operon termination sites in vitro.转录终止的合成位点及其与色氨酸操纵子终止位点在体外的功能比较。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4180-4. doi: 10.1073/pnas.78.7.4180.
2
Codon usage in bacteria: correlation with gene expressivity.细菌中的密码子使用：与基因表达能力的相关性
Nucleic Acids Res. 1982 Nov 25;10(22):7055-74. doi: 10.1093/nar/10.22.7055.
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Suppression of the ssb-1 and ssb-113 mutations of Escherichia coli by a wild-type rep gene, NaCl, and glucose.
Mol Biol Rep. 2010 Oct;37(7):3165-70. doi: 10.1007/s11033-009-9896-5. Epub 2009 Oct 13.
4
UvrD303, a hyperhelicase mutant that antagonizes RecA-dependent SOS expression by a mechanism that depends on its C terminus.UvrD303，一种超解旋酶突变体，通过一种依赖其C端的机制拮抗RecA依赖的SOS表达。
J Bacteriol. 2009 Mar;191(5):1429-38. doi: 10.1128/JB.01415-08. Epub 2008 Dec 12.
5
Viability of rep recA mutants depends on their capacity to cope with spontaneous oxidative damage and on the DnaK chaperone protein.rep recA突变体的生存能力取决于它们应对自发氧化损伤的能力以及DnaK伴侣蛋白。
J Bacteriol. 2001 Apr;183(7):2165-71. doi: 10.1128/JB.183.7.2165-2171.2001.
6
Essential role for the Legionella pneumophila rep helicase homologue in intracellular infection of mammalian cells.嗜肺军团菌解旋酶同源物在哺乳动物细胞胞内感染中的重要作用。
Infect Immun. 2000 Dec;68(12):6970-8. doi: 10.1128/IAI.68.12.6970-6978.2000.
7
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
8
Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency.具有提高解旋效率的大肠杆菌DNA解旋酶II突变体的鉴定与表征
J Bacteriol. 1998 Jan;180(2):377-87. doi: 10.1128/JB.180.2.377-387.1998.
9
Effects of lysine-to-glycine mutations in the ATP-binding consensus sequences in the AddA and AddB subunits on the Bacillus subtilis AddAB enzyme activities.枯草芽孢杆菌AddAB酶中AddA和AddB亚基的ATP结合共有序列中赖氨酸到甘氨酸突变对酶活性的影响。
J Bacteriol. 1996 Sep;178(17):5130-7. doi: 10.1128/jb.178.17.5130-5137.1996.
10
The C terminus of the AddA subunit of the Bacillus subtilis ATP-dependent DNase is required for the ATP-dependent exonuclease activity but not for the helicase activity.枯草芽孢杆菌ATP依赖型DNA酶的AddA亚基的C末端是ATP依赖型核酸外切酶活性所必需的，但不是解旋酶活性所必需的。
J Bacteriol. 1996 Sep;178(17):5086-91. doi: 10.1128/jb.178.17.5086-5091.1996.
野生型阻遏基因、氯化钠和葡萄糖对大肠杆菌ssb - 1和ssb - 113突变的抑制作用
J Bacteriol. 1982 Nov;152(2):572-83. doi: 10.1128/jb.152.2.572-583.1982.
4
Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.缓冲液梯度凝胶和35S标记辅助快速DNA序列测定。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5. doi: 10.1073/pnas.80.13.3963.
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A DNA sequence analysis package for the IBM personal computer.一款用于IBM个人计算机的DNA序列分析软件包。
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Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli.大肠杆菌dnaB的核苷酸序列及dnaB蛋白的一级结构
J Biol Chem. 1984 Jan 10;259(1):97-101.