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Characterization of DNA helicase II from a uvrD252 mutant of Escherichia coli.

作者信息

Washburn B K, Kushner S R

机构信息

Department of Genetics, University of Georgia, Athens 30602.

出版信息

J Bacteriol. 1993 Jan;175(2):341-50. doi: 10.1128/jb.175.2.341-350.1993.

DOI:10.1128/jb.175.2.341-350.1993
PMID:8419285
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC196147/
Abstract

The loss of DNA helicase II (UvrD) in Escherichia coli results in sensitivity to UV light and increased levels of spontaneous mutagenesis. While the effects of various uvrD alleles have been analyzed in vivo, the proteins produced by these alleles have not been examined in any detail. We have cloned one of these alleles, uvrD252, and determined the site of the mutation conferring the phenotype. In addition, the protein it encodes has been purified to homogeneity and characterized in vitro. The mutation responsible for the phenotype was identified as a glycine-to-aspartic-acid change in the putative ATP-binding domain. In comparison to wild-type DNA helicase II, the UvrD252 enzyme exhibited reduced levels of ATPase activity and a large increase in the Km for ATP. The ability of UvrD252 to unwind DNA containing single-stranded regions, as well as DNA containing only nicks, was reduced in comparison to that of the wild-type enzyme. Possible interpretations of these results in relation to the phenotypes of the uvrD252 mutant are discussed. This represents the first detailed analysis of the biochemical properties of a mutant DNA helicase II protein.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb83/196147/e69646ab8111/jbacter00044-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb83/196147/a5f23181c776/jbacter00044-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb83/196147/e69646ab8111/jbacter00044-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb83/196147/a5f23181c776/jbacter00044-0050-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb83/196147/e69646ab8111/jbacter00044-0054-a.jpg

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引用本文的文献

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Active displacement of RecA filaments by UvrD translocase activity.通过UvrD解旋酶活性实现RecA丝的主动位移。
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3
Resolving Holliday junctions with Escherichia coli UvrD helicase.用大肠杆菌 UvrD 解旋酶解决 Holliday 连接点。

本文引用的文献

1
ISOLATION AND CHARACTERIZATION OF RECOMBINATION-DEFICIENT MUTANTS OF ESCHERICHIA COLI K12.大肠杆菌K12重组缺陷突变体的分离与鉴定
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Mutator mutations in Escherichia coli induced by the insertion of phage mu and the transposable resistance elements Tn5 and Tn10.由噬菌体mu以及转座抗性元件Tn5和Tn10的插入所诱导的大肠杆菌中的诱变突变。
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Suppression of constitutive SOS expression by recA4162 (I298V) and recA4164 (L126V) requires UvrD and RecX in Escherichia coli K-12.在大肠杆菌K-12中，recA4162（I298V）和recA4164（L126V）对组成型SOS表达的抑制需要UvrD和RecX。
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UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli.在大肠杆菌的一个rep突变体中，解旋酶UvrD和UvrD252会对抗RecQ、RecJ和RecFOR。
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UvrD controls the access of recombination proteins to blocked replication forks.解旋酶UvrD控制重组蛋白接近受阻的复制叉。
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The DNA repair helicase UvrD is essential for replication fork reversal in replication mutants.DNA修复解旋酶UvrD对于复制突变体中的复制叉逆转至关重要。
EMBO Rep. 2004 Oct;5(10):983-8. doi: 10.1038/sj.embor.7400262. Epub 2004 Sep 17.
8
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
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9
Identification and characterization of Escherichia coli DNA helicase II mutants that exhibit increased unwinding efficiency.具有提高解旋效率的大肠杆菌DNA解旋酶II突变体的鉴定与表征
J Bacteriol. 1998 Jan;180(2):377-87. doi: 10.1128/JB.180.2.377-387.1998.
10
Conserved motifs II to VI of DNA helicase II from Escherichia coli are all required for biological activity.来自大肠杆菌的DNA解旋酶II的保守基序II至VI对于生物活性都是必需的。
J Bacteriol. 1997 Dec;179(23):7544-50. doi: 10.1128/jb.179.23.7544-7550.1997.
大肠杆菌紫外线敏感型uvrD3、recL152和uvrE15突变体的表型
Mutat Res. 1981 Aug;83(1):49-59. doi: 10.1016/0027-5107(81)90070-1.
4
Hyper-recombination in uvrD mutants of Escherichia coli K-12.大肠杆菌K-12的uvrD突变体中的超重组
Mol Gen Genet. 1980;180(1):185-91. doi: 10.1007/BF00267368.
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Effect of the uvrD mutation on excision repair.uvrD突变对切除修复的影响。
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A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
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The nucleotide sequence of the uvrD gene of E. coli.大肠杆菌uvrD基因的核苷酸序列。
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8
Long repair replication patches are produced by the short-patch pathway in a uvrD252 (recL152) mutant of Escherichia coli K-12.在大肠杆菌K-12的uvrD252（recL152）突变体中，长修复复制补丁是由短补丁途径产生的。
J Bacteriol. 1984 May;158(2):749-53. doi: 10.1128/jb.158.2.749-753.1984.
9
The Escherichia coli uvrD gene is inducible by DNA damage.大肠杆菌uvrD基因可被DNA损伤诱导。
Mol Gen Genet. 1983;191(3):397-400. doi: 10.1007/BF00425753.
10
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