Tokunaga M, Wada N, Hishinuma F
Nucleic Acids Res. 1987 Feb 11;15(3):1031-46. doi: 10.1093/nar/15.3.1031.
The linear dsDNA plasmids, pGKL1 (8.9 kb) and pGKL2 (13.4 kb) discovered in Kluyveromyces lactis, confer killer and immunity characteristics upon various yeast strains. We have devised an immunity assay and have been able to show the expression of an immunity phenotype in the K. lactis transformants harbouring conventional circular plasmids which contain DNA fragments of pGKL1. Using this expression system, the immunity determinant on pGKL1 was identified as ORF5. In addition, the presence of pGKL2 was proved to be essential for the expression of the immunity phenotype. This is the first demonstration of this new pGKL2 function, as distinct from its known functions for the replication and maintenance of pGKL1 in yeast cells.
在乳酸克鲁维酵母中发现的线性双链DNA质粒pGKL1（8.9 kb）和pGKL2（13.4 kb）赋予了多种酵母菌株杀伤和免疫特性。我们设计了一种免疫测定方法，并能够在携带含有pGKL1 DNA片段的传统环状质粒的乳酸克鲁维酵母转化子中显示免疫表型的表达。利用这个表达系统，pGKL1上的免疫决定簇被鉴定为ORF5。此外，已证明pGKL2的存在对于免疫表型的表达至关重要。这是pGKL2这种新功能的首次证明，不同于其在酵母细胞中对pGKL1进行复制和维持的已知功能。
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