Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 37673, Korea.
School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 37673, Korea.
J Ind Microbiol Biotechnol. 2019 Oct;46(9-10):1313-1326. doi: 10.1007/s10295-019-02191-5. Epub 2019 May 27.
Evolutionary approaches have been providing solutions to various bioengineering challenges in an efficient manner. In addition to traditional adaptive laboratory evolution and directed evolution, recent advances in synthetic biology and fluidic systems have opened a new era of evolutionary engineering. Synthetic genetic circuits have been created to control mutagenesis and enable screening of various phenotypes, particularly metabolite production. Fluidic systems can be used for high-throughput screening and multiplexed continuous cultivation of microorganisms. Moreover, continuous directed evolution has been achieved by combining all the steps of evolutionary engineering. Overall, modern tools and systems for evolutionary engineering can be used to establish the artificial equivalent to natural evolution for various research applications.
进化方法一直以高效的方式为各种生物工程挑战提供解决方案。除了传统的适应性实验室进化和定向进化，合成生物学和流体系统的最新进展开创了进化工程的新时代。已经创建了合成遗传电路来控制诱变并实现各种表型的筛选，特别是代谢产物的生产。流体系统可用于高通量筛选和微生物的多路连续培养。此外，通过组合进化工程的所有步骤，可以实现连续定向进化。总的来说，进化工程的现代工具和系统可用于为各种研究应用建立类似于自然进化的人工进化。
