洗牌子：质粒R64的四个相邻DNA片段的多次倒位产生七个不同的开放阅读框。
Shufflon: multi-inversion of four contiguous DNA segments of plasmid R64 creates seven different open reading frames.
作者信息
Komano T, Kubo A, Nisioka T
出版信息
Nucleic Acids Res. 1987 Feb 11;15(3):1165-72. doi: 10.1093/nar/15.3.1165.
Abstract
The IncI alpha plasmid R64 was found to bear a highly mobile DNA segment which was designated as a clustered inversion region (J. Bacteriol. 165, 94-100, 1986). The clustered inversion region consists of four DNA segments designated respectively as A, B, C and D which differ in molecular size and restriction sites. The four DNA segments invert independently or in groups resulting in a complex DNA rearrangement. We now show the nucleotide sequence of the clustered inversion region of R64. The present results suggest that the clustered inversion region is a biological switch to select one of seven open reading frames whose primary structures at the region proximal to N-termini are constant while those at the C-terminal region are variable. A name, "Shufflon" was proposed to call this kind of the clustered inversion region.
摘要
发现IncIα质粒R64带有一个高度可移动的DNA片段，该片段被指定为成簇倒位区域（《细菌学杂志》165, 94 - 100, 1986）。成簇倒位区域由四个分别命名为A、B、C和D的DNA片段组成，它们在分子大小和限制酶切位点上有所不同。这四个DNA片段可独立或成组地发生倒位，导致复杂的DNA重排。我们现在展示了R64成簇倒位区域的核苷酸序列。目前的结果表明，成簇倒位区域是一个生物开关，用于选择七个开放阅读框中的一个，这些开放阅读框在靠近N端的区域一级结构是恒定的，而在C端区域则是可变的。有人提出用 “Shufflon” 这个名字来称呼这种成簇倒位区域。
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