Huang Yue, Su Shuhua, Duan Bo, Zhang Yunfei, Xu Zhengmin
Department of Otolaryngology-Head and Neck Surgery, Children's Hospital of Fudan University, No 399 Wanyuan Road, Shanghai, China.
Department of Otolaryngology, Qinzhou Maternity and Child Health Care Hospital, Qinzhou, Guangxi Province, China.
Open Life Sci. 2025 Mar 28;20(1):20221030. doi: 10.1515/biol-2022-1030. eCollection 2025.
Jaranol, a bioactive compound derived from various traditional medicinal herbs, has demonstrated significant anti-inflammatory properties. This study investigates its effects and possible mechanisms underlying its anti-inflammatory role in mast cells, as well as ovalbumin (OVA)-induced allergic rhinitis (AR) mice model. Forty mice were randomly divided into blank, AR, dexamethasone (positive control), and Jaranol groups (10 mg/ml), with 10 mice in each group. Jaranol was found to inhibit nasal mucosal inflammation and reduce mast cell numbers in AR models. It also inhibited the secretion of several inflammatory cytokines (IFN-γ, TNF-α, IL-1β, IL-6, MCP-1, and CXCR10) from mast cells, as well as mast cell proliferation and migration. Interestingly, Pirin was differentially expressed in blank, AR, and Jaranol groups. Further studies indicated that Jaranol inhibited inflammatory cytokine secretion from mast cells by mediating Pirin and also inhibited mast cell proliferation and migration. Moreover, it inhibited mast cell function by suppressing Pirin expression. These findings suggest that Jaranol exerts its therapeutic effects by inhibiting Pirin expression in mast cells, thereby reducing inflammation and histopathological changes associated with AR.
Jaranol是一种从多种传统草药中提取的生物活性化合物，已显示出显著的抗炎特性。本研究调查了其在肥大细胞以及卵清蛋白（OVA）诱导的变应性鼻炎（AR）小鼠模型中的抗炎作用及其潜在机制。40只小鼠随机分为空白组、AR组、地塞米松（阳性对照组）和Jaranol组（10mg/ml），每组10只。研究发现Jaranol可抑制AR模型中的鼻黏膜炎症并减少肥大细胞数量。它还抑制肥大细胞分泌多种炎性细胞因子（IFN-γ、TNF-α、IL-1β、IL-6、MCP-1和CXCR10），以及肥大细胞的增殖和迁移。有趣的是，Pirin在空白组、AR组和Jaranol组中存在差异表达。进一步研究表明，Jaranol通过介导Pirin抑制肥大细胞炎性细胞因子的分泌，还抑制肥大细胞的增殖和迁移。此外，它通过抑制Pirin表达来抑制肥大细胞功能。这些发现表明，Jaranol通过抑制肥大细胞中Pirin的表达发挥其治疗作用，从而减轻与AR相关的炎症和组织病理学变化。
