Miller-Edge M, Splitter G
Vet Immunol Immunopathol. 1986 Dec;13(4):301-19. doi: 10.1016/0165-2427(86)90024-3.
Lymphocyte proliferation was used to evaluate T cell-mediated immune responses to different isolates of Bovine Herpesvirus 1 (BHV-1). Groups of high, moderate, and low responses were observed when lymphocytes from three breeds of dairy cattle were stimulated with each of the BHV-1 isolates. Proliferation of cells in the low responding group could be augmented by exogenous IL-2. The mechanism of unresponsiveness by cells from one individual whose response was not altered by IL-2 supplementation was further investigated. The patterns of response by limiting dilution frequency analysis eliminated the possibility that this individual lacked responsive cells but suggested the presence of regulatory cell interactions which resulted in the observed low proliferative response. These results show that animals exposed to the same environment can vary greatly in their ability to respond to BHV-1. At least two mechanisms may be responsible for low proliferative responses in vitro: inadequate levels of IL-2 and the presence of suppressor cells.
淋巴细胞增殖被用于评估T细胞介导的对不同牛疱疹病毒1型（BHV-1）分离株的免疫反应。当用每种BHV-1分离株刺激来自三个奶牛品种的淋巴细胞时，观察到高、中、低反应组。低反应组细胞的增殖可通过外源性白细胞介素-2（IL-2）增强。对一名个体的细胞无反应机制进行了进一步研究，该个体的反应不受IL-2补充的影响。通过有限稀释频率分析的反应模式排除了该个体缺乏反应性细胞的可能性，但表明存在调节性细胞相互作用，导致观察到的低增殖反应。这些结果表明，暴露于相同环境的动物对BHV-1的反应能力可能有很大差异。体外低增殖反应可能至少由两种机制引起：IL-2水平不足和抑制细胞的存在。
