Pashmakova Medora B, Barr James W, Bishop Micah A
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843.
Am J Vet Res. 2015 May;76(5):420-5. doi: 10.2460/ajvr.76.5.420.
To compare stability of hemostatic proteins in canine fresh-frozen plasma (FFP) thawed with a modified commercial microwave warmer (MCM) or warm water bath (37°C; WWB) or at room temperature (22°C).
Fresh-frozen plasma obtained from 8 canine donors of a commercial blood bank.
A commercial microwave warmer was modified with a thermocouple to measure surface temperature of bags containing plasma. The MCM and a WWB were each used to concurrently thaw a 60-mL bag of plasma obtained from the same donor. Two 3-mL control aliquots of FFP from each donor were thawed to room temperature without use of a heating device. Concentrations of hemostatic proteins, albumin, and D-dimers; prothrombin time (PT); and activated partial thromboplastin time (aPTT) were determined for all samples.
Significant decreases in concentrations of factors II, IX, X, XI, fibrinogen, von Willebrand factor, antithrombin, protein C, and albumin and significant increases in PT and aPTT were detected for plasma thawed with the MCM, compared with results for samples thawed with the WWB. Concentrations of factors VII, VIII, and XII were not significantly different between plasma thawed with the MCM and WWB. Concentrations of D-dimers were above the reference range for all thawed samples regardless of thawing method. No significant differences in factor concentrations were detected between control and WWB-thawed samples.
Significant differences in hemostatic protein concentrations and coagulation times were detected for plasma thawed with an MCM but not between control and WWB-thawed samples. Clinical importance of these changes should be investigated.
比较使用改良商用微波加热器（MCM）、温水浴（37°C；WWB）或室温（22°C）解冻的犬新鲜冷冻血浆（FFP）中止血蛋白的稳定性。
从一家商业血库的8只犬供血者获取的新鲜冷冻血浆。
用热电偶对商用微波加热器进行改良，以测量装有血浆的袋子表面温度。MCM和WWB分别用于同时解冻从同一供血者获得的一袋60 mL血浆。每个供血者的两份3 mL FFP对照等分试样在不使用加热设备的情况下解冻至室温。测定所有样本中止血蛋白、白蛋白和D - 二聚体的浓度；凝血酶原时间（PT）；以及活化部分凝血活酶时间（aPTT）。
与用WWB解冻的样本结果相比，用MCM解冻的血浆中检测到因子II、IX、X、XI、纤维蛋白原、血管性血友病因子、抗凝血酶蛋白C和白蛋白浓度显著降低，PT和aPTT显著升高。用MCM和WWB解冻的血浆中因子VII、VIII和XII的浓度无显著差异。无论解冻方法如何，所有解冻样本的D - 二聚体浓度均高于参考范围。对照样本和用WWB解冻的样本之间未检测到因子浓度的显著差异。
用MCM解冻的血浆在止血蛋白浓度和凝血时间方面存在显著差异，但对照样本和用WWB解冻的样本之间无差异。应研究这些变化的临床重要性。
