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用于输血的微波解冻犬血浆的评估。

Evaluation of microwave-thawed canine plasma for transfusion.

作者信息

Hurst T S, Turrentine M A, Johnson G S

出版信息

J Am Vet Med Assoc. 1987 Apr 1;190(7):863-5.

PMID:3570940
Abstract

Units of fresh-frozen canine plasma were thawed rapidly in a microwave oven, using a mean of 15.4 thawing periods of 10 seconds each. The activated partial thromboplastin times, the one-stage prothrombin times, concentrations of fibrinogen, factor-VIII coagulant activity, and von Willebrand factor antigen of microwave-thawed units were not significantly different from those measured in small aliquots of the same units thawed in a warm water bath (n = 5). Five dogs were given a unit of their own microwave-thawed plasma. During the 24 hours after infusion, no significant changes were measured in temperature, heart rate, or respiration rate. In addition, significant changes in PCV, total protein concentrations, estimates of platelet numbers, total RBC counts, total WBC counts, and differential WBC counts were not measured in blood specimens obtained before infusion and 24 hours after the infusion of microwave-thawed plasma.

摘要

将新鲜冷冻犬血浆单位在微波炉中快速解冻,平均使用15.4个解冻周期,每个周期10秒。微波解冻单位的活化部分凝血活酶时间、一期凝血酶原时间、纤维蛋白原浓度、因子VIII促凝活性和血管性血友病因子抗原与在温水浴中解冻的相同单位的小份样本所测得的结果无显著差异(n = 5)。给5只狗输注其自身微波解冻的血浆单位。在输注后的24小时内,体温、心率或呼吸频率均未测得显著变化。此外,在输注微波解冻血浆前和输注后24小时采集的血液样本中,红细胞压积、总蛋白浓度、血小板数量估计值、红细胞总数、白细胞总数和白细胞分类计数均未测得显著变化。

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