Laboratory of Atherothrombosis, Moscow State University of Medicine and Dentistry, Moscow, Russia.
Ariel University, Institute for Translational Research, Ariel, Israel.
Thromb Haemost. 2018 Nov;118(11):1969-1981. doi: 10.1055/s-0038-1673342. Epub 2018 Oct 9.
Acute myocardial infarction (AMI) is associated with activation of various cells, including platelets that form monocyte-platelet complexes (MPCs). Here, we analysed MPC in vivo and in vitro and investigated the abilities of different monocyte subclasses to form MPC, the characteristics of the cells involved in MPC formation and MPC changes in AMI. We identified MPC by co-staining for platelet antigen CD41a and monocyte antigens CD14 and CD16. Platelet activation was evaluated from expression of phosphatidylserine as revealed by annexin V. Our results confirm published data and provide new information regarding the patterns of MPC in AMI patients. We found that the patterns of platelet aggregation with monocytes were different in AMI patients and controls: (1) in AMI patients, MPC formed by intermediate monocytes carry more platelets whereas in healthy controls more platelets aggregated with classical monocytes; (2) the numbers of MPC in AMI patients, being already higher than in controls, were further increased if these patients suffered various in-hospital complications; (3) on the basis of the CD41a fluorescence of the antibody-stained MPC, some of the aggregates seem to consist of monocytes and platelet-derived extracellular vesicles (EVs); (4) aggregation of monocytes with platelet EV occurred in in vitro experiments; and (5) these experiments demonstrated that monocytes from AMI patients aggregate with both platelets and platelet EVs more efficiently than do monocytes from controls. MPC in AMI patients may play an important role in this pathology.
急性心肌梗死（AMI）与各种细胞的激活有关，包括形成单核细胞-血小板复合物（MPC）的血小板。在这里，我们分析了体内和体外的 MPC，并研究了不同单核细胞亚群形成 MPC 的能力、参与 MPC 形成的细胞的特征以及 AMI 中 MPC 的变化。我们通过同时染色血小板抗原 CD41a 和单核细胞抗原 CD14 和 CD16 来识别 MPC。通过用 Annexin V 揭示的血小板活化来评估血小板的激活。我们的结果证实了已发表的数据，并提供了有关 AMI 患者 MPC 模式的新信息。我们发现，AMI 患者和对照组中血小板与单核细胞聚集的模式不同：（1）在 AMI 患者中，中间单核细胞形成的 MPC 携带更多的血小板，而在健康对照组中，更多的血小板与经典单核细胞聚集；（2）AMI 患者的 MPC 数量已经高于对照组，如果这些患者患有各种院内并发症，则会进一步增加；（3）基于抗体染色的 MPC 的 CD41a 荧光，一些聚集物似乎由单核细胞和血小板衍生的细胞外囊泡（EVs）组成；（4）在体外实验中观察到单核细胞与血小板 EV 的聚集；（5）这些实验表明，与对照组的单核细胞相比，AMI 患者的单核细胞与血小板和血小板 EV 聚集的效率更高。AMI 患者中的 MPC 可能在这种病理中发挥重要作用。
