Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, Department for Health Sciences and Biomedicine, Danube University Krems, Dr.-Karl-Dorrek-Strasse 30, 3500, Krems, Austria.
Core Facility Imaging, Medical University of Vienna, Lazarettgasse 14, 1090, Vienna, Austria.
Sci Rep. 2018 Apr 26;8(1):6598. doi: 10.1038/s41598-018-25047-x.
Secretion and exchange of biomolecules via extracellular vesicles (EVs) are crucial mechanisms in intercellular communication, and the roles of EVs in infection, inflammation, or thrombosis have been increasingly recognized. EVs have emerged as central players in immune regulation and can enhance or suppress the immune response, depending on the state of donor and recipient cells. We investigated the interaction of blood cell-derived EVs with leukocyte subpopulations (monocytes and their subsets, granulocytes, B cells, T cells, and NK cells) directly in whole blood using a combination of flow cytometry, imaging flow cytometry, cell sorting, and high resolution confocal microscopy. Platelet-derived EVs constituted the majority of circulating EVs and were preferentially associated with granulocytes and monocytes, while they scarcely interacted with lymphocytes. Further flow cytometric differentiation of monocyte subsets provided clear indications for a preferential association of platelet-derived EVs with intermediate (CD14CD16) monocytes in whole blood.
细胞外囊泡（EVs）通过分泌和交换生物分子在细胞间通讯中起着至关重要的作用，EVs 在感染、炎症或血栓形成中的作用也越来越受到重视。EVs 已成为免疫调节的核心参与者，可根据供体细胞和受体细胞的状态增强或抑制免疫反应。我们使用流式细胞术、成像流式细胞术、细胞分选和高分辨率共聚焦显微镜直接在全血中研究了血细胞衍生的 EV 与白细胞亚群（单核细胞及其亚群、粒细胞、B 细胞、T 细胞和 NK 细胞）的相互作用。血小板衍生的 EV 构成了循环 EV 的大部分，并且优先与粒细胞和单核细胞相关，而与淋巴细胞几乎没有相互作用。对单核细胞亚群的进一步流式细胞术分化清楚地表明，血小板衍生的 EV 优先与全血中的中间（CD14+CD16+）单核细胞相关联。
