He Rui-Han, Liang Qi-Yun, Zhao Jin-Xin, Du Zong-Jun
1College of Marine Science, Shandong University, Weihai 264209, PR China.
2Infection and Immunity Program, Monash Biomedicine Discovery Institute, and Department of Microbiology, Monash University, Melbourne 3800, Australia.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3647-3651. doi: 10.1099/ijsem.0.003053. Epub 2018 Oct 10.
A Gram-stain-negative, rod-shaped, non-motile, aerobic and orange-pigmented marine bacterium, designated strain L53, was isolated from an oyster sample collected from the coast of Weihai, China (122.0° E 37.5° N). Growth of strain L53 occurred at 4-40 °C (optimum, 33 °C), at pH 6.5-8.0 (optimum, pH 7.5) and with 1.0-7.0 % (w/v) NaCl (optimum, 3.0 %). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain L53 was closely related to Bizionia echini KCTC 22015 (96.9 %) and Hanstruepera neustonica JCM 19743 (96.1 %). Strain L53 was located in a distinct phyletic lineage in a discrete clade associated with H. neustonica JCM 19743. The DNA G+C content of strain L53 was 33.5 mol%. The sole menaquinone was MK-6. The polar lipids comprised one phosphatidylethanolamine, four unidentified aminolipids and four unidentified lipids. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 0 G. Based on morphological, physiological and molecular properties as well as on phylogenetic distinctiveness, strain L53 should be placed in the genus Hanstruepera as representing a novel species, for which the name Hanstruepera crassostreae sp. nov. is proposed. The type strain is L53 (=KCTC 62247=MCCC 1H00246).
从中国威海海岸（东经122.0°，北纬37.5°）采集的牡蛎样本中分离出一株革兰氏染色阴性、杆状、无运动性、需氧且产橙色色素的海洋细菌，命名为L53菌株。L53菌株在4-40 °C（最适温度33 °C）、pH 6.5-8.0（最适pH 7.5）和1.0-7.0 %（w/v）NaCl（最适浓度3.0 %）条件下生长。基于16S rRNA基因序列的系统发育分析表明，L53菌株与刺海胆毕氏菌KCTC 22015（相似度96.9 %）和海洋汉氏菌JCM 19743（相似度96.1 %）密切相关。L53菌株位于与海洋汉氏菌JCM 19743相关的一个离散分支中的一个独特系统发育谱系中。L53菌株的DNA G+C含量为33.5 mol%。唯一的甲基萘醌是MK-6。极性脂质包括一种磷脂酰乙醇胺、四种未鉴定的氨基脂质和四种未鉴定的脂质。主要脂肪酸为异C15 : 0、异C17 : 0 3-OH和异C15 : 0 G。基于形态、生理和分子特性以及系统发育独特性，L53菌株应归入汉氏菌属，代表一个新物种，为此提出汉氏厚壳贻贝菌新种Hanstruepera crassostreae sp. nov.。模式菌株为L53（=KCTC 62247=MCCC 1H00246）。
