Hefei National Laboratory for Physical Sciences at Microscale, Department of Materials Science and Engineering, University of Science and Technology of China, The Anhui Key Laboratory of Condensed Matter Physics at Extreme Conditions, High Magnetic Field Laboratory, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230026, P. R. China.
School of Life Sciences, University of Science and Technology of China, Hefei, 230026, P. R. China.
Adv Healthc Mater. 2018 Oct;7(19):e1800322. doi: 10.1002/adhm.201800322. Epub 2018 Aug 13.
Multifunctional theranostic nanoagents which realize precise diagnosis and treatment of tumors are attracting increasing interests in recent years. However, efficient and controlled synthesis of ultra-small noble metal nanoagents remains a challenge. Here, monodisperse Gd/Ru@BSA nanodots (GRBNDs) are successfully fabricated via a totally "green", "one-pot" protocol for in situ reduction of Ru(III) and biomineralization of Gd(III) in the presence of albumin. The as-prepared nanoagent possesses the features of being ultra small in size (≈6.7 nm), having strong colloidal stability, and thermal stability as well as high photothermal conversion efficiency (η = 50.7%). As expected, the GRBNDs achieve a significant efficacy of anticancer therapy under LASER activation both in vitro and in vivo. It also exhibits superior T -weighted magnetic resonance (MR) imaging ability due to its high longitudinal relaxivity value (r = 10.98 × 10 m s ). Moreover, it is demonstrated to be renal clearable with negligible systemic toxicity. This work highlights a straightforward and repeatable approach for synthesizing highly effective and multifunctional noble metal nanoagent of great clinical promising for cancer theranostics.
近年来，能够实现肿瘤精确诊断和治疗的多功能治疗性纳米制剂引起了越来越多的关注。然而，高效且可控的超小贵金属纳米制剂的合成仍然是一个挑战。在此，通过在白蛋白存在下的原位还原 Ru(III)和 Gd(III)的生物矿化，成功地制备了单分散的 Gd/Ru@BSA 纳米点（GRBNDs）。所制备的纳米制剂具有超小尺寸（≈6.7nm）、强胶体稳定性和热稳定性以及高光热转换效率（η=50.7%）的特点。正如预期的那样，在 LASER 激活下，GRBNDs 在体外和体内均实现了显著的抗癌治疗效果。由于其具有较高的纵向弛豫率值（r=10.98×10m s），因此它还表现出优异的 T1 加权磁共振（MR）成像能力。此外，研究表明它具有可被肾脏清除的特性，并且具有可忽略的全身毒性。这项工作突出了一种简单且可重复的方法，用于合成高效且多功能的贵金属纳米制剂，为癌症的治疗提供了很大的临床应用前景。
