Vilela Thais C, Leffa Daniela D, Damiani Adriani P, Damazio Daiane Dal Col, Manenti Aline V, Carvalho Tiago José G, Ramlov Fernanda, Amaral Patricia A, Andrade Vanessa M DE
Translational Biomedicine Laboratory, Graduate Programme of Health Sciences, Health Sciences Unit, University of Southern Santa Catarina (UNESC), 1105, Universitária Rd, 88806-000 Criciúma, SC, Brazil.
Laboratory of Plant Morphogenesis and Biochemistry, Department of Botany, Federal University of Santa Catarina (UFSC), Eng. Agronômico Andrei Cristian Ferreira Rd, Trindade, 88040-900 Florianópolis, SC, Brazil.
An Acad Bras Cienc. 2018 Jul-Sep;90(3):3165-3174. doi: 10.1590/0001-3765201820180144.
Hibiscus acetosella was shown to exert beneficial effects in humans and animal models however, the effects of this plant on DNA are unknown. The aim of this study was to determine the antigenotoxic and antimutagenic effects of H. acetosella extracts on alkylating agent methyl methanesulfonate (MMS) in vivo in mice. Initially, we performed analysis of phenolic compounds in extracts of H. acetosella by high-performance liquid chromatography (HPLC). Next, mice were divided into 8 groups and treated with distilled water or plant extract (0.1 ml/10 g) by gavage for 15 days, followed by intraperitoneal (ip) administration of saline solution or MMS (40 mg/Kg b.w) on day 16. Caffeic acid, following by gallic acid, gallocatechin, coumaric acid, and 3,4-dihydroxybenzoic acid were found to be present in extracts of H. acetosella leaves. In peripheral blood analysis of groups receiving pretreatment with H. acetosella at doses of 50 or 100 mg/kg plus MMS decreased DNA damage as evidenced by comet assay and Micronucleus assays relative to MMS alone. These results suggested that H. acetosella extracts exerted protective effects dose dependent against genotoxicity and mutagenicity induced by alkylating agents.
已证明红叶槿对人类和动物模型有有益作用，然而，这种植物对DNA的影响尚不清楚。本研究的目的是确定红叶槿提取物对小鼠体内烷基化剂甲磺酸甲酯（MMS）的抗遗传毒性和抗诱变作用。最初，我们通过高效液相色谱（HPLC）对红叶槿提取物中的酚类化合物进行了分析。接下来，将小鼠分为8组，通过灌胃给予蒸馏水或植物提取物（0.1 ml/10 g），持续15天，然后在第16天腹腔注射生理盐水或MMS（40 mg/Kg体重）。发现红叶槿叶提取物中存在咖啡酸，其次是没食子酸、表儿茶素、香豆酸和3,4-二羟基苯甲酸。在彗星试验和微核试验中，相对于单独使用MMS，接受50或100 mg/kg剂量红叶槿预处理加MMS的组在外周血分析中DNA损伤减少。这些结果表明，红叶槿提取物对烷基化剂诱导的遗传毒性和诱变性具有剂量依赖性的保护作用。
