用于干细胞筛选的基于磁性的细胞分离技术

Magnetic-Based Cell Isolation Technique for the Selection of Stem Cells.

作者信息

Korkusuz Petek, Köse Sevil, Yersal Nilgün, Önen Selin

机构信息

Hacettepe University, Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey.

Atilim University, Faculty of Health Sciences, Department of Nutrition and Dietetics, Ankara, Turkey.

出版信息

Methods Mol Biol. 2019;1879:153-163. doi: 10.1007/7651_2018_151.

DOI:10.1007/7651_2018_151

PMID:30306535

Abstract

Magnetic-activated cell sorting (MACS) is the technology that is recently used as a magnetic-based cell isolation/purification technique. This technique enables the isolation and selection of germ, hematopoietic, and somatic stem cells including skin stem cells (SkSCs). Here, we have tried to describe the isolation of stem cells by MACS using CD34 antigen for SkSCs, again CD34 for hematopoietic stem cells (HSCs) and Thy-1 for spermatogonial stem cells (SpSCs). MACS allowed the isolation of CD34+, CD34+, and Thy-1+ human SkSCs, HSCs, and SpSCs with minimum 98% purity.

摘要

磁激活细胞分选（MACS）是一种最近被用作基于磁性的细胞分离/纯化技术。该技术能够分离和选择包括皮肤干细胞（SkSCs）在内的生殖、造血和体干细胞。在此，我们试图描述通过MACS使用针对SkSCs的CD34抗原、针对造血干细胞（HSCs）的CD34以及针对精原干细胞（SpSCs）的Thy-1来分离干细胞的方法。MACS能够以至少98%的纯度分离出CD34+、CD34+和Thy-1+的人SkSCs、HSCs和SpSCs。