Department of Basic Sciences Pharmacy, Institute of Health Sciences, Van Yuzuncu Yil University, Van, Turkey.
Department of Biochemistry, Faculty of Pharmacy, Van Yuzuncu Yil University, Van, 650080, Turkey.
BMC Complement Med Ther. 2023 Nov 14;23(1):410. doi: 10.1186/s12906-023-04252-w.
Scutellaria Pinnatifida subsp. pichleri (Stapf) Rech.f. (SP) is used in folk medicine for the treatment of diabetes. The aim of the study was to determine the phenolic profile of SP extract (SPE) by LC-MS/MS and to investigate the antidiabetic, hepatoprotective and nephroprotective effects of SPE in streptozotosin (STZ)-induced diabetic rat model.
Forty-two rats were randomly divided into six groups (n = 7): Control (nondiabetic), diabetes mellitus (DM), DM + SP-100 (diabetic rats treated with SPE, 100 mg/kg/day), DM + SP-200 (diabetic rats treated with SPE, 200 mg/kg/day), DM + SP-400 (diabetic rats treated with SPE, 400 mg/kg/day) and DM + Gly-3 (diabetic rats treated with glibenclamide, 3 mg/kg/day). Live body weight, fasting blood glucose (FBG) level, antidiabetic, serum biochemical and lipid profile parameters, antioxidant defense system, malondyaldehyde (MDA) and histopathological examinations in liver, kidney and pancreas were evaluated.
Apigenin, luteolin, quinic acid, cosmosiin and epigallocatechin were determined to be the major phenolic compounds in the SPE. Administration of the highest dose of SP extract (400 mg/kg) resulted in a significant reduction in FBG levels and glycosylated hemoglobin levels in STZ-induced diabetic rats, indicating an antihyperglycemic effect. SPE (200 and 400 mg/kg) and glibenclamide significantly improved MDA in liver and kidney tissues. In addition, SPE contributed to the struggle against STZ-induced oxidative stress by stimulating antioxidant defense systems. STZ induction negatively affected liver, kidney and pancreas tissues according to histopathological findings. Treatment with 400 mg/kg and glibenclamide attenuated these negative effects.
In conclusion, the extract of the aerial part of Scutellaria pinnatifida subsp. pichleri has hepatoprotective, nephroprotective and insulin secretion stimulating effects against STZ-induced diabetes and its complications due to its antidiabetic and antioxidant phytochemicals such as apigenin, luteolin, quinic acid, cosmosiin and epigallocatechin.
黄芩亚种。pichleri（Stapf）Rech.f.（SP）在民间医学中用于治疗糖尿病。本研究的目的是通过 LC-MS/MS 来确定 SP 提取物（SPE）的酚类成分，并研究 SPE 在链脲佐菌素（STZ）诱导的糖尿病大鼠模型中的抗糖尿病、肝保护和肾保护作用。
42 只大鼠随机分为六组（n=7）：对照组（非糖尿病）、糖尿病（DM）、DM+SP-100（糖尿病大鼠用 SPE 治疗，100mg/kg/天）、DM+SP-200（糖尿病大鼠用 SPE 治疗，200mg/kg/天）、DM+SP-400（糖尿病大鼠用 SPE 治疗，400mg/kg/天）和 DM+Gly-3（糖尿病大鼠用格列本脲治疗，3mg/kg/天）。评估活体重、空腹血糖（FBG）水平、降糖、血清生化和血脂谱参数、抗氧化防御系统、丙二醛（MDA）和肝、肾和胰腺的组织病理学检查。
在 SPE 中确定了芹菜素、木樨草素、奎宁酸、 cosmosiin 和表没食子儿茶素没食子酸酯为主要酚类化合物。SP 提取物的最高剂量（400mg/kg）给药可显著降低 STZ 诱导的糖尿病大鼠的 FBG 水平和糖化血红蛋白水平，表明具有降血糖作用。SPE（200 和 400mg/kg）和格列本脲显著改善了肝和肾组织中的 MDA。此外，SPE 通过刺激抗氧化防御系统有助于对抗 STZ 诱导的氧化应激。根据组织病理学发现，STZ 诱导对肝、肾和胰腺组织产生负面影响。400mg/kg 和格列本脲的治疗减轻了这些负面影响。
总之，黄芩亚种的地上部分提取物具有抗糖尿病和抗氧化植物化学物质如芹菜素、木樨草素、奎宁酸、 cosmosiin 和表没食子儿茶素没食子酸酯，对 STZ 诱导的糖尿病及其并发症具有肝保护、肾保护和刺激胰岛素分泌作用。
