a Yong Researchers and Elite Club , Islamic Azad University , Jolfa , Iran.
b Stem Cell Research Center , Tabriz University of Medical Sciences , Tabriz , Iran.
Artif Cells Nanomed Biotechnol. 2018;46(sup3):S911-S921. doi: 10.1080/21691401.2018.1518911. Epub 2018 Oct 11.
Iron oxide nanoparticles (IONs) have been extensively applied in cancer therapy and theranostics due to their admissible magnetic properties, excellent chemical stability and biocompatibility. Herein, a novel stimuli-responsive magnetic nanocomposite was synthesized for cancer therapy; thereby, the triblock copolymer of poly[(2-succinyloxyethylmethacrylate)-b-(N-isopropylacrylamide)-b-dimethylaminoethylmethacrylate) [poly(SEMA-b-NIPAM-b-DMAEMA)] was prepared by reversible addition of fragmentation chain transfer (RAFT) polymerization. This triblock copolymer with carboxylic groups of succinyloxyethylmethacrylate was adsorbed onto the surface of FeO nanoparticles. The morphology, nanocomposite properties and stimuli-responsive behaviours were investigated by field emission scanning electron microscopy, X-ray diffraction, dynamic light scattering, vibrating sample magnetometer (VSM) and thermogravimetric analysis. Doxorubicin (DOX) encapsulation efficacy was 94.3%. Release behaviours of DOX from the magnetic nanocomposite exhibited that the rate of DOX release could be efficiently controlled through temperature and pH. The cytotoxicity of the drug was investigated in vitro against breast cancer cell line (MCF7) using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assays, 4',6-diamidino-2-phenylindole (DAPI) staining and cellular uptake. In conclusion, the synthesized DOX@nanocomposite can be applied in theranostic applications and anticancer drug delivery owing to admissible properties.
氧化铁纳米粒子（IONs）由于其可接受的磁性、优异的化学稳定性和生物相容性，已被广泛应用于癌症治疗和治疗学。在此，合成了一种新型的刺激响应磁性纳米复合材料用于癌症治疗；因此，通过可逆加成断裂链转移（RAFT）聚合制备了聚[（2-琥珀酰氧基乙基甲基丙烯酸酯）-b-（N-异丙基丙烯酰胺）-b-二甲基氨基乙基甲基丙烯酸酯）[聚（SEMA-b-NIPAM-b-DMAEMA）]嵌段共聚物。这种带有琥珀酰氧基乙基甲基丙烯酸的羧酸基团的三嵌段共聚物被吸附到 FeO 纳米粒子的表面。通过场发射扫描电子显微镜、X 射线衍射、动态光散射、振动样品磁强计（VSM）和热重分析研究了形貌、纳米复合材料性能和刺激响应行为。阿霉素（DOX）包封效率为 94.3%。通过温度和 pH 值可以有效地控制 DOX 从磁性纳米复合材料中的释放行为。通过（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物）（MTT）测定法、4'，6-二脒基-2-苯吲哚（DAPI）染色和细胞摄取，在体外研究了药物对乳腺癌细胞系（MCF7）的细胞毒性。总之，由于具有可接受的性质，合成的 DOX@纳米复合材料可应用于治疗学和抗癌药物输送。
