School of Applied Biological Science, Hiroshima University, Higashi-Hiroshima, Japan.
Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Japan.
PLoS One. 2018 Oct 11;13(10):e0205522. doi: 10.1371/journal.pone.0205522. eCollection 2018.
TRYPTICHON (TRY) and ENHANCER OF TRY AND CPC2 (ETC2) encode R3-type MYB transcription factors that are involved in epidermal cell differentiation in Arabidopsis thaliana. TRY and ETC2 belong to the CPC-like MYB gene family, which includes seven homolog genes. Previously, we showed that among the CPC family members, TRY and ETC2 are characterized by rapid proteolysis compared with that of other members, and we demonstrated that this proteolysis is mediated by the proteasome-dependent pathway. In this study, we compared the functions of the wild-type TRY and ETC2 proteins and their amino acid-substituted versions. Our results showed that the substitution of amino acids in the C-terminal of TRY and ETC2 conferred them the ability to induce root hair formation. Furthermore, we confirmed that these mutations enhanced the stability of the TRY and ETC2 proteins. These results revealed that the amino acids, which are important for the functions of TRY and ETC2, mediate morphological pattern formation and can be useful in understanding the pathway determining the fate of root hair cells.
TRYPTICHON（TRY）和增强子的TRY 和 CPC2（ETC2）编码 R3 型 MYB 转录因子，参与拟南芥表皮细胞分化。TRY 和 ETC2 属于 CPC 样 MYB 基因家族，包括七个同源基因。以前，我们表明，在 CPC 家族成员中，TRY 和 ETC2 的特点是与其他成员相比，快速蛋白水解，我们证明这种蛋白水解是由蛋白酶体依赖性途径介导的。在这项研究中，我们比较了野生型 TRY 和 ETC2 蛋白及其氨基酸取代版本的功能。我们的结果表明，TRY 和 ETC2 的 C 末端氨基酸取代赋予它们诱导根毛形成的能力。此外，我们证实这些突变增强了 TRY 和 ETC2 蛋白的稳定性。这些结果表明，TRY 和 ETC2 的功能的重要氨基酸介导形态模式形成，并且可以在理解决定根毛细胞命运的途径中是有用的。
