Garcia Thiago Alves, Tamura Ozaki Guilherme Akio, Castoldi Robson Chacon, Koike Tatiana Emy, Trindade Camargo Regina Celi, Silva Camargo Filho José Carlos
Universidade Estadual de Campinas (UNICAMP), Programa de Pós-graduação em Ciências da Cirurgia, Campinas, SP, Brazil.
Universidade Estadual de Campinas (UNICAMP), Programa de Pós-graduação em Ciências da Cirurgia, Campinas, SP, Brazil.
Tissue Cell. 2018 Oct;54:120-126. doi: 10.1016/j.tice.2018.08.014. Epub 2018 Sep 5.
To evaluate alterations from different therapies in muscular injury using the Fractal Dimension (FD) method.
35 animals were allocated in Control Group (C), Injury Control Group (IC), Injury Low Level Laser Therapy Group (ILT), Injury Platelet Rich Plasma Group (IP), and Injury LLLT and PRP Group (ILP). The animals suffered a stretch injury in gastrocnemius muscle and after that IP and ILP groups received PRP application. The ILT and ILP groups received daily LLLT applications for seven days. After seven days the animals were euthanized and the gastrocnemius muscle removed and frozen. The muscles were stained with Hematoxylin and Eosin (HE) and Picrosirius Red, for observation of the morphology of the injury and semi-quantitative and quantitative analysis through the Fractal Dimension (FD) method.
In the qualitative and semi-quantitative analysis, in relation to IC group, the ILT presented a reduction in rounded fibers and the IP in angular fibers. The ILP group demonstrated a reduction in both polymorphic fibers and inflammatory infiltrate. The FD of the muscles stained with HE was higher in the groups that suffered the injury when compared to the C group (p < 0.05); the FD of the collagen demonstrated no statistical difference between the groups.
Both treatments were able to accelerate injury repair, and the association of both presented better results than the isolated applications. However, the FD method showed no sensitivity to differentiate the treatments, either in the histological aspects or the injury in collagen.
使用分形维数（FD）方法评估不同疗法对肌肉损伤的影响。
将35只动物分为对照组（C）、损伤对照组（IC）、损伤低强度激光治疗组（ILT）、损伤富血小板血浆组（IP）和损伤低强度激光治疗与富血小板血浆联合组（ILP）。动物的腓肠肌遭受拉伸损伤，之后IP组和ILP组接受富血小板血浆注射。ILT组和ILP组每天接受低强度激光治疗，持续7天。7天后对动物实施安乐死，取出腓肠肌并冷冻。肌肉用苏木精和伊红（HE）以及天狼星红染色，用于观察损伤形态，并通过分形维数（FD）方法进行半定量和定量分析。
在定性和半定量分析中，与IC组相比，ILT组圆形纤维减少，IP组角形纤维减少。ILP组多形纤维和炎性浸润均减少。与C组相比，遭受损伤的组中HE染色肌肉的FD更高（p < 0.05）；各组之间胶原蛋白的FD无统计学差异。
两种治疗均能加速损伤修复，且联合治疗比单独应用效果更好。然而，FD方法在组织学方面或胶原蛋白损伤方面对区分治疗方法均不敏感。
