Schoolof Science and Technology, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK.
Food Chem. 2019 Jan 30;272:117-125. doi: 10.1016/j.foodchem.2018.08.036. Epub 2018 Aug 9.
This study aimed to investigate the cytoprotective effects of flavonoids, their metabolites alone or in combination against hypoxia/reoxygenation induced oxidative stress in the transformed human first trimester trophoblast cell line (HTR-8/SVneo). Oxidative stress was achieved with hypoxia followed by reoxygenation and the following assays were performed: MTT, CellTox™ Green Cytotoxicity, CellTiter-Glo®, NADP/NADPH-Glo™, ROS-Glo™/HO, GSH/GSSG-Glo™ and Caspase-Glo® 3/7 assays. HTR-8/SVneo cells, pre-treated for 24 h with flavonoids or their metabolites were protected significantly from oxidative stress. Flavonoids were associated with ROS modulation, reducing the generation of superoxide/hydrogen peroxide. The activities of caspases 3/7 were also significantly reduced significantly in HTR-8/SVneo cells pre-treated with flavonoids. This study has shown for the first time that 24 h pre-treatment with flavonoids, their metabolites alone or in combination, protected against HR-induced oxidative stress in the trophoblast cell line. These data indicate that dietary flavonoids may be beneficial to placental health and invasion during early gestation.
本研究旨在探讨黄酮类化合物及其单独或联合代谢物对人早孕滋养层细胞系（HTR-8/SVneo）缺氧/复氧诱导的氧化应激的细胞保护作用。通过缺氧后复氧来实现氧化应激，并进行以下检测：MTT、CellTox™ Green 细胞毒性检测、CellTiter-Glo®、NADP/NADPH-Glo™、ROS-Glo™/HO、GSH/GSSG-Glo™和 Caspase-Glo® 3/7 检测。用黄酮类化合物或其代谢物预处理 24 小时的 HTR-8/SVneo 细胞，可显著抵抗氧化应激。黄酮类化合物与 ROS 调节有关，可减少超氧阴离子/过氧化氢的产生。用黄酮类化合物预处理的 HTR-8/SVneo 细胞中，caspase 3/7 的活性也显著降低。本研究首次表明，黄酮类化合物及其代谢物单独或联合预处理 24 小时可防止滋养层细胞系 HR 诱导的氧化应激。这些数据表明，饮食中的黄酮类化合物可能有益于早期妊娠期间胎盘的健康和侵袭。
