• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

综合代谢组学和转录组学分析不同发育阶段韩城大红袍果皮（花椒）中类黄酮生物合成的调控机制。

Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis.

机构信息

Northwest Agriculture and Forestry University, College of Science, Yangling, 712100, China.

Forestry and Grassland Bureau of Xunhua Salar autonomous county, Xunhua, 811100, China.

出版信息

BMC Plant Biol. 2022 May 21;22(1):251. doi: 10.1186/s12870-022-03642-5.

DOI:10.1186/s12870-022-03642-5
PMID:35596133
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9123719/
Abstract

BACKGROUND

Flavonoids have strong free radical scavenging and antioxidant capacity. The high abundance of flavonoids in Chinese prickly ash peels have many benefits to human health. In this study, 'Hancheng Dahongpao', a main cultivar, was taken as materials to investigate the flavonoids biosynthesis mechanism of Zanthoxylum bungeanum Maxim at three key development stages by integration of metabolomics and transcriptomics analysis.

RESULTS

A total of 19 differentially accumulated metabolites were identified, the key flavonoids compounds were kaempferol, quercetin and their glycoside derivatives, and two major anthocyanins (peonidin O-hexoside and peonidin 3-O-glucoside). 5 gene networks/modules including 15 important candidate genes were identified, which was highly correlated with flavonoids. Among these genes, ZM-163828 and ZM-184209 were strongly correlated with kaempferol and quercetin, and ZM-125833 and ZM-97481 were controlled the anthocyanins biosynthesis. Moreover, it was shown that MYB-ZM1, MYB-ZM3, MYB-ZM5, MYB-ZM6 and MYB-ZM7 coordinately controlled flavonoids accumulation through regulating the structural genes.

CONCLUSIONS

Generally, this study systematically revealed the flavonoids metabolic pathways and candidate genes involved in flavonoids biosynthesis and laid a foundation for the potential targets for the breeding of new valuable Chinese prickly ash cultivars.

摘要

背景

类黄酮具有很强的自由基清除和抗氧化能力。花椒果皮中类黄酮含量丰富，对人体健康有诸多益处。本研究以主要栽培品种“韩城大红袍”为材料，通过代谢组学和转录组学分析，综合研究了花椒果实发育三个关键时期类黄酮的生物合成机制。

结果

共鉴定出 19 个差异积累代谢物，关键类黄酮化合物为山奈酚、槲皮素及其糖苷衍生物，以及两种主要的花色苷（矢车菊素 O-己糖苷和矢车菊素 3-O-葡萄糖苷）。鉴定出包括 15 个重要候选基因在内的 5 个基因网络/模块，与类黄酮高度相关。其中，ZM-163828 和 ZM-184209 与山奈酚和槲皮素的相关性较强，ZM-125833 和 ZM-97481 控制花色苷的生物合成。此外，研究表明，MYB-ZM1、MYB-ZM3、MYB-ZM5、MYB-ZM6 和 MYB-ZM7 通过调节结构基因协同调控类黄酮的积累。

结论

本研究系统揭示了花椒果实发育过程中类黄酮代谢途径及参与类黄酮生物合成的候选基因，为花椒新的有价值品种的选育提供了潜在目标。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/62affa65c6c2/12870_2022_3642_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/c9f4eaa5eb30/12870_2022_3642_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/488e3ca16301/12870_2022_3642_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/e4888c4bd65b/12870_2022_3642_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/94759a51bfa9/12870_2022_3642_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/c9e2517577c5/12870_2022_3642_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/2c487f973238/12870_2022_3642_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/62affa65c6c2/12870_2022_3642_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/c9f4eaa5eb30/12870_2022_3642_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/488e3ca16301/12870_2022_3642_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/e4888c4bd65b/12870_2022_3642_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/94759a51bfa9/12870_2022_3642_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/c9e2517577c5/12870_2022_3642_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/2c487f973238/12870_2022_3642_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3247/9123719/62affa65c6c2/12870_2022_3642_Fig7_HTML.jpg

相似文献

1
Regulation mechanisms of flavonoids biosynthesis of Hancheng Dahongpao peels (Zanthoxylum bungeanum Maxim) at different development stages by integrated metabolomics and transcriptomics analysis.综合代谢组学和转录组学分析不同发育阶段韩城大红袍果皮（花椒）中类黄酮生物合成的调控机制。
BMC Plant Biol. 2022 May 21;22(1):251. doi: 10.1186/s12870-022-03642-5.
2
Transcriptome and metabolome analyses reveal the regulation of peel coloration in green, red Chinese prickly ash ( L.).转录组和代谢组分析揭示了青、红两种颜色的中国花椒（Zanthoxylum bungeanum Maxim.）果皮着色的调控机制。
Food Chem (Oxf). 2020 Oct 22;1:100004. doi: 10.1016/j.fochms.2020.100004. eCollection 2020 Oct 2.
3
Comparative Transcriptome Analysis and Expression of Genes Reveal the Biosynthesis and Accumulation Patterns of Key Flavonoids in Different Varieties of Leaves.比较转录组分析和基因表达揭示了不同品种叶片中关键类黄酮的生物合成和积累模式。
J Agric Food Chem. 2019 Dec 4;67(48):13258-13268. doi: 10.1021/acs.jafc.9b05732. Epub 2019 Nov 21.
4
Time-series based metabolomics reveals the characteristics of the color-related metabolites during the different coloration stages of Zanthoxylum bungeanum peel.基于时间序列的代谢组学揭示了花椒果皮不同着色阶段与颜色相关代谢物的特征。
Food Res Int. 2022 May;155:111077. doi: 10.1016/j.foodres.2022.111077. Epub 2022 Feb 28.
5
Transcriptomics integrated with metabolomics to characterize key pigment compounds and genes related to anthocyanin biosynthesis in Zanthoxylum bungeanum peel.转录组学与代谢组学相结合以表征花椒果皮中与花青素生物合成相关的关键色素化合物和基因。
Physiol Plant. 2023 Sep-Oct;175(5):e14031. doi: 10.1111/ppl.14031.
6
Integrated Transcriptome and Metabolome Analysis Revealed That Flavonoid Biosynthesis May Dominate the Resistance of against Stem Canker.整合转录组和代谢组分析表明，类黄酮生物合成可能主导了 对茎溃疡病的抗性。
J Agric Food Chem. 2021 Jun 9;69(22):6360-6378. doi: 10.1021/acs.jafc.1c00357. Epub 2021 May 27.
7
Insights into the catalytic and regulatory mechanisms of dihydroflavonol 4-reductase, a key enzyme of anthocyanin synthesis in Zanthoxylum bungeanum.花椒花青素合成关键酶二氢黄酮醇4-还原酶的催化和调控机制研究
Tree Physiol. 2023 Jan 5;43(1):169-184. doi: 10.1093/treephys/tpac101.
8
Metabolomics and transcriptomics provide insights into the flavonoid biosynthesis pathway in the roots of developing Aster tataricus.代谢组学和转录组学为研究紫菀属植物根中类黄酮生物合成途径提供了新视角。
J Plant Res. 2023 Jan;136(1):139-156. doi: 10.1007/s10265-022-01426-4. Epub 2022 Dec 15.
9
Comparative metabolomics analysis of pericarp from four varieties of Maxim.四种益智果皮的比较代谢组学分析
Bioengineered. 2022 Jun;13(6):14815-14826. doi: 10.1080/21655979.2022.2108632.
10
Quality evaluation of different varieties of Zanthoxylum bungeanum Maxim. peels based on phenolic profiles, bioactivity, and HPLC fingerprint.基于酚类成分、生物活性和 HPLC 指纹图谱评价不同品种花椒果皮的质量。
J Food Sci. 2020 Apr;85(4):1090-1097. doi: 10.1111/1750-3841.15095. Epub 2020 Mar 9.

引用本文的文献

1
Study on the dynamics of fruits color related genes and metabolites during the fruit development of Zanthoxylum bungeanum 'Hanyuan'.汉源花椒果实发育过程中果实颜色相关基因和代谢产物的动态研究
BMC Plant Biol. 2025 Jul 26;25(1):960. doi: 10.1186/s12870-025-06962-4.
2
Roles of MARCKSL1, MCM6, RFC4, and PLAU genes in esophageal cancer and their association with radiotherapy response.MARCKSL1、MCM6、RFC4和PLAU基因在食管癌中的作用及其与放疗反应的关联。
Sci Rep. 2025 Jul 2;15(1):23559. doi: 10.1038/s41598-025-08528-8.
3
Optimization of ultrasonic-assisted extraction of total flavonoids from residue by response surface methodology and evaluation of its algicidal properties.

本文引用的文献

1
Transcriptome and metabolome analyses reveal the regulation of peel coloration in green, red Chinese prickly ash ( L.).转录组和代谢组分析揭示了青、红两种颜色的中国花椒（Zanthoxylum bungeanum Maxim.）果皮着色的调控机制。
Food Chem (Oxf). 2020 Oct 22;1:100004. doi: 10.1016/j.fochms.2020.100004. eCollection 2020 Oct 2.
2
Chemotaxonomic variation in volatile component contents and their correlation between climate factors in Chinese prickly ash peels ( Maxim.).花椒（Maxim.）果皮挥发性成分含量的化学分类学变异及其与气候因子的相关性。
Food Chem X. 2021 Dec 1;12:100176. doi: 10.1016/j.fochx.2021.100176. eCollection 2021 Dec 30.
3
响应面法优化超声辅助从残渣中提取总黄酮及其杀藻性能评价
Front Microbiol. 2025 Mar 13;16:1540631. doi: 10.3389/fmicb.2025.1540631. eCollection 2025.
4
Extraction and purification of total flavonoids from Zanthoxylum planispinum Var. Dintanensis leaves and effect of altitude on total flavonoids content.齿瓣石山花椒叶总黄酮的提取纯化及海拔对总黄酮含量的影响
Sci Rep. 2025 Feb 27;15(1):7080. doi: 10.1038/s41598-025-91528-5.
5
Molecular and Metabolic Regulation of Flavonoid Biosynthesis in Two Varieties of .两种……中黄酮类生物合成的分子与代谢调控
Curr Issues Mol Biol. 2024 Dec 18;46(12):14270-14290. doi: 10.3390/cimb46120855.
6
Unveiling the Molecular Mechanisms of Browning in Callus through Transcriptomic and Metabolomic Analysis.通过转录组学和代谢组学分析揭示愈伤组织中棕色化的分子机制。
Int J Mol Sci. 2024 Oct 14;25(20):11021. doi: 10.3390/ijms252011021.
7
Research advances of polyphenols in inflammatory diseases.多酚类物质在炎症性疾病中的研究进展。
Front Immunol. 2024 Jan 26;15:1305886. doi: 10.3389/fimmu.2024.1305886. eCollection 2024.
8
Integrative metabolome and transcriptome analyses reveal the coloration mechanism in Camellia oleifera petals with different color.综合代谢组学和转录组学分析揭示了不同颜色的油茶花瓣的着色机制。
BMC Plant Biol. 2024 Jan 2;24(1):19. doi: 10.1186/s12870-023-04699-6.
9
Multi-environment evaluations across ecological regions reveal climate and soil effects on amides contents in Chinese prickly ash peels (Zanthoxylum bungeanum Maxim.).多环境评估跨越生态区域揭示了气候和土壤对中国花椒果皮酰胺含量的影响。
BMC Plant Biol. 2023 Jun 13;23(1):313. doi: 10.1186/s12870-023-04328-2.
10
Integrated transcriptomics and metabolomics analysis provide insight into anthocyanin biosynthesis for sepal color formation in .综合转录组学和代谢组学分析为[具体植物名称]萼片颜色形成中的花青素生物合成提供了见解。 （你提供的原文中“in”后面缺少具体内容，这里补充了“[具体植物名称]”以便完整理解句子意思）
Front Plant Sci. 2023 Feb 20;14:1044581. doi: 10.3389/fpls.2023.1044581. eCollection 2023.
Identification and quantification of target metabolites combined with transcriptome of two rheum species focused on anthraquinone and flavonoids biosynthesis.
鉴定和定量两种大黄属植物的目标代谢物，并结合转录组分析，重点研究蒽醌和黄酮类化合物的生物合成。
Sci Rep. 2020 Nov 20;10(1):20241. doi: 10.1038/s41598-020-77356-9.
4
Flavonoids and Their Metabolites: Prevention in Cardiovascular Diseases and Diabetes.黄酮类化合物及其代谢产物：对心血管疾病和糖尿病的预防作用
Diseases. 2017 Sep 5;5(3):19. doi: 10.3390/diseases5030019.
5
Integrated Metabolomics and Transcriptomics Analyses Reveal the Molecular Mechanisms Underlying the Accumulation of Anthocyanins and Other Flavonoids in Cowpea Pod ( L.).整合代谢组学和转录组学分析揭示豇豆荚中花色苷和其他类黄酮积累的分子机制。
J Agric Food Chem. 2020 Aug 26;68(34):9260-9275. doi: 10.1021/acs.jafc.0c01851. Epub 2020 Aug 13.
6
Quality evaluation of different varieties of Zanthoxylum bungeanum Maxim. peels based on phenolic profiles, bioactivity, and HPLC fingerprint.基于酚类成分、生物活性和 HPLC 指纹图谱评价不同品种花椒果皮的质量。
J Food Sci. 2020 Apr;85(4):1090-1097. doi: 10.1111/1750-3841.15095. Epub 2020 Mar 9.
7
Dynamics of antioxidant activities, metabolites, phenolic acids, flavonoids, and phenolic biosynthetic genes in germinating Chinese wild rice (Zizania latifolia).萌发过程中中国野生稻（Zizania latifolia）抗氧化活性、代谢产物、酚酸、类黄酮和酚类生物合成基因的动态变化。
Food Chem. 2020 Jul 15;318:126483. doi: 10.1016/j.foodchem.2020.126483. Epub 2020 Feb 25.
8
Transcriptome and metabolome profiling unveil the mechanisms of Ziziphus jujuba Mill. peel coloration.转录组和代谢组谱分析揭示了枣果皮颜色形成的机制。
Food Chem. 2020 May 15;312:125903. doi: 10.1016/j.foodchem.2019.125903. Epub 2019 Nov 14.
9
Antioxidative effects of flavonoids and their metabolites against hypoxia/reoxygenation-induced oxidative stress in a human first trimester trophoblast cell line.黄酮类化合物及其代谢物对人早孕绒毛滋养层细胞株缺氧/复氧诱导氧化应激的抗氧化作用。
Food Chem. 2019 Jan 30;272:117-125. doi: 10.1016/j.foodchem.2018.08.036. Epub 2018 Aug 9.
10
Anthocyanins accumulation and molecular analysis of correlated genes by metabolome and transcriptome in green and purple asparaguses (Asparagus officinalis, L.).绿芦笋和紫芦笋（石刁柏，L.）中花色苷积累及代谢组学和转录组学相关基因的分子分析。
Food Chem. 2019 Jan 15;271:18-28. doi: 10.1016/j.foodchem.2018.07.120. Epub 2018 Jul 18.