Department of Traditional Chinese Medicine, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Department of Obstetrics and Gynecology, Second Affiliated Hospital, Medical School of Xi'an Jiaotong University, China.
Life Sci. 2020 Sep 1;256:117890. doi: 10.1016/j.lfs.2020.117890. Epub 2020 Jun 1.
We aim to investigate whether there is activation of NLRP1 and autophagy in trophoblast oxidative stress model. Resveratrol was taken to clarify its role in oxidative damage of placental trophoblasts.
HO was added to HTR-8/SVneo cell for 3 h, then the ROS level and apoptosis panel was performed. The levels of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 were detected. Resveratrol was added after 8 h, the ROS level and apoptosis rate were detected, the expression of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 were detected.
300 μmol/L HO for 3 h is the optimum combination in establishing the oxidative stress injury model (P < 0.01). LDH, ROS and MDA level was increased, the activity of SOD, CAT were declined (P < 0.01). Apoptosis rate increased (P < 0.01). The expression of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 protein was higher (P < .01). Resveratrol (50 μmol/L) treatment for 8 h could improve the changes caused by HO, increase the survival rate of cells (P < 0.01), reduce the release of LDH, decrease the level of MDA, increase the level of SOD and CAT (P < 0.01). The expression of IL-1β, caspase-1, NLRP1, LC3 and Beclin-1 protein decreased (P < 0.01).
Trophoblast oxidative damage model can be established under 300 μmol/L HO for 3 h, the expression of NLRP1and autophagy after HO treatment were detected. Resveratrol reduces apoptotic cells, thus ensuring the normal biological functions of trophoblasts.
HO-induced oxidative stress damage model in HTR-8/SVneo cells can be successfully established under 300 μmol/L HO for 3 h, resveratrol alleviates of HO-induced damage by its antioxidant and autophagy regulation function.
研究 NLRP1 和自噬是否在滋养层氧化应激模型中被激活。用白藜芦醇来阐明其在胎盘滋养层氧化损伤中的作用。
在 HTR-8/SVneo 细胞中加入 HO 3 小时，然后进行 ROS 水平和凋亡检测。检测 IL-1β、caspase-1、NLRP1、LC3 和 Beclin-1 的水平。8 小时后加入白藜芦醇，检测 ROS 水平和凋亡率，检测 IL-1β、caspase-1、NLRP1、LC3 和 Beclin-1 的表达。
300 μmol/L HO 作用 3 小时是建立氧化应激损伤模型的最佳组合（P < 0.01）。LDH、ROS 和 MDA 水平升高，SOD、CAT 活性下降（P < 0.01）。凋亡率增加（P < 0.01）。IL-1β、caspase-1、NLRP1、LC3 和 Beclin-1 蛋白表达升高（P < 0.01）。白藜芦醇（50 μmol/L）处理 8 小时可改善 HO 引起的变化，提高细胞存活率（P < 0.01），降低 LDH 释放，降低 MDA 水平，增加 SOD 和 CAT 水平（P < 0.01）。IL-1β、caspase-1、NLRP1、LC3 和 Beclin-1 蛋白表达降低（P < 0.01）。
在 300 μmol/L HO 作用 3 小时下可建立滋养层氧化损伤模型，检测 HO 处理后 NLRP1 和自噬的表达。白藜芦醇通过抗氧化和自噬调节功能减轻 HO 诱导的损伤，减少凋亡细胞，从而保证滋养层的正常生物学功能。
在 300 μmol/L HO 作用 3 小时下可成功建立 HTR-8/SVneo 细胞 HO 诱导的氧化应激损伤模型，白藜芦醇通过其抗氧化和自噬调节功能缓解 HO 诱导的损伤。
