Yang Hainan, Liu Zaijia, Li Xufeng, Zhang Zhenfeng, Chen Deji, Lian Hui
Department of Radiology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, China.
Department of Medical Image, Ezhou Central Hospital, Ezhou, 436000, China.
Nanoscale Res Lett. 2018 Oct 11;13(1):319. doi: 10.1186/s11671-018-2700-5.
Herein, a tumor-targeted multifunctional theranostic agent was synthetized using a facile method, combining four clinically approved materials: artesunate (Arte), human serum albumin (HSA), folic acid (FA), and indocyanine green (ICG). The obtained nanocomposites (FA-IHA NPs) showed an excellent photo- and physiological stability. The ICG in the FA-IHA NPs was used not only for near infrared (NIR) fluorescence imaging, but also for photothermal and photodynamic (PTT-PDT) therapy under a single NIR irradiation. In addition, the NIR irradiation (808 nm, 1 W/cm) could trigger Arte release that showed enhanced chemotherapeutic effect. Through fluorescence imaging, the cell uptake and tumor accumulation of FA-IHA NPs were observed in vitro and in vivo, analyzed by confocal microscopy and NIR fluorescence imaging in tumor xenograft mice. Based on the diagnostic results, FA-IHA NPs at 24 h post injection and combined with NIR irradiation (808 nm, 1 W/cm) could efficiently suppress tumor growth through a photo-chemo combination therapy, with no tumor recurrence in vitro and in vivo. The obtained results suggested that FA-IHA NPs are promising photo-chemo theranostic agents for future clinical translation.
在此，我们采用一种简便的方法合成了一种肿瘤靶向多功能诊疗剂，该方法将四种临床批准的材料结合在一起：青蒿琥酯（Arte）、人血清白蛋白（HSA）、叶酸（FA）和吲哚菁绿（ICG）。所制备的纳米复合材料（FA-IHA NPs）表现出优异的光稳定性和生理稳定性。FA-IHA NPs中的ICG不仅用于近红外（NIR）荧光成像，还用于在单一近红外照射下的光热和光动力（PTT-PDT）治疗。此外，近红外照射（808 nm，1 W/cm）可触发青蒿琥酯释放，显示出增强的化疗效果。通过荧光成像，在体外和体内观察了FA-IHA NPs的细胞摄取和肿瘤蓄积，并通过共聚焦显微镜和肿瘤异种移植小鼠的近红外荧光成像进行分析。基于诊断结果，注射后24小时的FA-IHA NPs与近红外照射（808 nm，1 W/cm）相结合，可通过光化学联合疗法有效抑制肿瘤生长，在体外和体内均无肿瘤复发。所得结果表明，FA-IHA NPs是未来临床转化中很有前景的光化学诊疗剂。
