Department of Chemistry, Capital Normal University, China.
Department of Cardiology, Coronary Heart Disease Center, State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Nov 15;1100-1101:122-130. doi: 10.1016/j.jchromb.2018.10.005. Epub 2018 Oct 7.
This urinary metabonomic study aimed to identify the potential metabolic biomarkers in acute coronary syndrome (ACS) patients. Ultra-performance liquid chromatography/mass spectrometry (UPLC/MS) was used to analyze the urine samples from ACS patients and healthy controls. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were applied to characterizing the endogenous metabolites and potential biomarker, respectively. Among twenty biomarkers that functioned in nine metabolic pathways, nine biomarkers were found up-regulated significantly, including of isobutyryl‑l‑carnitine, 3‑methylglutarylcarnitine, cinnavalininate, l‑tryptophan, 3‑methyldioxyindole, palmitic acid, N4‑acetylaminobutanal, 3‑sulfino‑l‑alanine and S‑adenosyl‑l‑homocysteine. The other eleven biomarkers were showed down-regulated, including of l‑lactic acid, trigonelline, nicotinuric acid, l‑alanine, d‑alanyl‑d‑alanine, creatine, N4‑acetylaminobutanoate, glutathionyl spermidine, 5‑methoxytryptamine, kynurenic acid and xanthurenic acid. This study also implied that fatty acid metabolism, fatty acid β‑oxidation metabolism, amino acid metabolism and TCA cycle played important roles in ACS. Therefore, urinary metabolomics may improve the diagnosis efficacy of ACS and make it more accurate and comprehensive for ACS diagnosis.
本尿代谢组学研究旨在鉴定急性冠脉综合征（ACS）患者中的潜在代谢生物标志物。采用超高效液相色谱/质谱联用（UPLC/MS）技术分析 ACS 患者和健康对照者的尿液样本。主成分分析（PCA）和正交偏最小二乘判别分析（OPLS-DA）分别用于表征内源性代谢物和潜在的生物标志物。在 20 个参与 9 个代谢途径的生物标志物中，有 9 个标志物显著上调，包括异丁酰肉碱、3-甲基戊二酰肉碱、肉桂酸盐、色氨酸、3-甲氧基吲哚、棕榈酸、N4-乙酰氨基丁醛、3-磺基-l-丙氨酸和 S-腺苷-l-同型半胱氨酸。其他 11 个标志物则表现为下调，包括 l-乳酸、葫芦巴碱、烟酸、丙氨酸、d-丙氨酰-d-丙氨酸、肌酸、N4-乙酰氨基丁酸、谷胱甘肽亚精胺、5-甲氧基色胺、犬尿氨酸和黄尿酸。本研究还表明，脂肪酸代谢、脂肪酸β-氧化代谢、氨基酸代谢和 TCA 循环在 ACS 中发挥重要作用。因此，尿代谢组学可能提高 ACS 的诊断效果，使其对 ACS 的诊断更加准确和全面。
