School of Pharmacy, Minzu University of China, Beijing, P.R. China.
Key Laboratory of Ethnomedicine (MINZU University of China), Ministry of Education, Beijing P.R. China.
Nat Prod Res. 2020 Mar;34(5):651-658. doi: 10.1080/14786419.2018.1495638. Epub 2018 Oct 15.
Three novel dammarane-type saponins, 2,3,12,20(),24()-pentahydroxydammar-25-ene-3---D-glucopyranosyl-(1→2)--D-glucopyranosyl-20---D-glucopyranoside (, namely gypenoside J1), 2,3,12,20(),25-pentahydroxydammar-23-ene-3---D-glucopyranosyl-(1→2)--D-glucopyranosyl-20---D-glucopyranoside (, namely gypenoside J2) and 2,3,12,20(S)-tetrahydroxydammar-25-en-24-one-3---D-glucopyranosyl-(1→2)--D-glucopyranosyl-20---D-xylopyranosyl-(1→6)--D-glucopyranoside (, namely gypenoside J3) along with one known gypenoside (gypenoside LVII) were isolated from the aerial parts of using various chromatographic methods. Their structures were elucidated on the basis of IR, 1D- (H and C), 2D-NMR spectroscopy (HSQC, HMBC and COSY), and mass spectrometry (ESI-MS/MS). Their activity was tested using CCK-8 assay. These four compounds showed little anti-cancer activity with IC values more than 100 μM against four types of human cancer lines. The effects of them against HO-induced oxidative stress in human neuroblastoma SH-SY5Y cells were evaluated and they all showed potential neuroprotective effects with 3.64-18.16% higher cell viability than the HO-induced model group.
从 中分离得到了三种新型达玛烷型皂苷，分别为 2,3,12,20(),24()-五羟基达玛-25-烯-3---D-吡喃葡萄糖基-(1→2)--D-吡喃葡萄糖基-20---D-吡喃葡萄糖苷（，即绞股蓝皂苷 J1）、2,3,12,20(),25-五羟基达玛-23-烯-3---D-吡喃葡萄糖基-(1→2)--D-吡喃葡萄糖基-20---D-吡喃葡萄糖苷（，即绞股蓝皂苷 J2）和 2,3,12,20(S)-四羟基达玛-25-烯-24-酮-3---D-吡喃葡萄糖基-(1→2)--D-吡喃葡萄糖基-20---D-木吡喃糖基-(1→6)--D-吡喃葡萄糖苷（，即绞股蓝皂苷 J3），以及一种已知的绞股蓝皂苷（绞股蓝皂苷 LVII）。通过各种色谱方法从 中分离得到。基于 IR、1D-（H 和 C）、2D-NMR 光谱（HSQC、HMBC 和 COSY）和质谱（ESI-MS/MS）确定了它们的结构。通过 CCK-8 测定法测试了它们的活性。这四种化合物对四种类型的人癌细胞系的抗癌活性均较弱，IC 值均大于 100 μM。评估了它们对 HO 诱导的人神经母细胞瘤 SH-SY5Y 细胞氧化应激的影响，与 HO 诱导模型组相比，它们均表现出潜在的神经保护作用，细胞活力提高了 3.64-18.16%。
