双视图光声显微镜用于定量细胞核成像。
Dual-view photoacoustic microscopy for quantitative cell nuclear imaging.
出版信息
Opt Lett. 2018 Oct 15;43(20):4875-4878. doi: 10.1364/OL.43.004875.
Abstract
Optical-resolution photoacoustic microscopy (OR-PAM) is an emerging imaging modality for studying biological tissues. However, in conventional single-view OR-PAM, the lateral and axial resolutions-determined optically and acoustically, respectively-are highly anisotropic. In this Letter, we introduce dual-view OR-PAM to improve axial resolution, achieving three-dimensional (3D) resolution isotropy. We first use 0.5 μm polystyrene beads and carbon fibers to validate the resolution isotropy improvement. Imaging of mouse brain slices further demonstrates the improved resolution isotropy, revealing the 3D structure of cell nuclei in detail, which facilitates quantitative cell nuclear analysis.
摘要
光学分辨率光声显微镜（OR-PAM）是一种新兴的用于研究生物组织的成像模式。然而，在传统的单视图 OR-PAM 中，分别由光学和声学确定的横向和轴向分辨率具有高度各向异性。在本研究中，我们引入了双视图 OR-PAM 以提高轴向分辨率，从而实现了三维（3D）分辨率各向同性。我们首先使用 0.5μm 聚苯乙烯珠和碳纤维来验证分辨率各向同性的改善。对小鼠脑切片的成像进一步证明了分辨率各向同性的改善，详细显示了细胞核的 3D 结构，这有助于对细胞核进行定量分析。
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