Liu Xiaowei, Wong Terence T W, Shi Junhui, Ma Jun, Yang Qing, Wang Lihong V
Opt Lett. 2018 Feb 15;43(4):947-950. doi: 10.1364/OL.43.000947.
Photoacoustic microscopy (PAM) with ultraviolet (UV) laser illumination has recently been demonstrated as a promising tool that provides fast, label-free, and multilayered histologic imaging of human breast tissue. Thus far, the axial resolution has been determined ultrasonically. To enable optically defined axial resolution, we exploit the Grüneisen relaxation (GR) effect. By imaging mouse brain slices, we show that GRUV-PAM reveals detailed information about three-dimensional cell nuclear distributions and internal structures, which are important diagnostic features for cancers. Due to the nonlinear effect, GRUV-PAM also provides better contrast in images of cell nuclei.
最近，采用紫外（UV）激光照明的光声显微镜（PAM）已被证明是一种很有前景的工具，可对人体乳腺组织进行快速、无标记的多层组织学成像。到目前为止，轴向分辨率一直是通过超声测定的。为了实现光学定义的轴向分辨率，我们利用了格林艾森弛豫（GR）效应。通过对小鼠脑切片成像，我们表明GRUV - PAM揭示了有关三维细胞核分布和内部结构的详细信息，这些是癌症的重要诊断特征。由于非线性效应，GRUV - PAM在细胞核图像中也提供了更好的对比度。
