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[从同源疫源地检测对鼠疫微生物菌株有活性的鼠疫病原体抑制剂。氯化锂对检测抑制剂的影响]

[Detection of an inhibitor of the causative agent of plague active against strains of the plague microbe from an homologous focus. The effect of lithium chloride on detecting the inhibitor].

作者信息

Gramotina L I

出版信息

Antibiot Med Biotekhnol. 1987 Jan;32(1):36-8.

PMID:3032085
Abstract

A nutrient medium containing 0.7 per cent of lithium chloride was tested and Yersinia pestis inhibitor was detected in 83.3 per cent of the strains on the growth tufts of 8 indicator strains isolated from a homologous focus. Lowering the lithium chloride concentration in the medium up to 0.6 per cent promoted detection of the inhibitor in a larger number of the tested strains (93.3 per cent). But even on this medium none of the indicator strains formed overall growth tufts. The subcultures of the indicator strains resistant to lithium chloride formed overall growth tufts on agar plates with lithium chloride and were sensitive to the inhibitor of the tested strains from the central Caucasus. However, during storage they lost their sensitivity to the inhibitor which did not permit their use as the test cultures.

摘要

对含有0.7%氯化锂的营养培养基进行了测试,从同源疫源地分离出的8株指示菌株的生长簇上,在83.3%的菌株中检测到了鼠疫耶尔森菌抑制剂。将培养基中氯化锂浓度降至0.6%,能促使更多受试菌株(93.3%)检测到该抑制剂。但即便在这种培养基上,也没有一株指示菌株形成完整的生长簇。对氯化锂有抗性的指示菌株的传代培养物,在含氯化锂的琼脂平板上形成了完整的生长簇,并且对来自中高加索地区受试菌株的抑制剂敏感。然而,在保存过程中,它们失去了对该抑制剂的敏感性,这使得它们无法用作测试培养物。

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