Muid R E, Penfield A, Dale M M
Biochem Biophys Res Commun. 1987 Mar 13;143(2):630-7. doi: 10.1016/0006-291x(87)91400-8.
A specific diacylglycerol kinase inhibitor, at a concentration of 10(-5) M, consistently enhanced superoxide generation from human neutrophils stimulated with fMet-Leu-Phe, IgG, heat-aggregated IgG and opsonized zymosan. The concentration-response curve for fMet-Leu-Phe was displaced to the left and the maximum superoxide release was also consistently increased by R59022 whereas the diacylglycerol lipase inhibitor, RHC80267, 10(-5) M, had no significant effect. These results suggest that the diacylglycerol formed after fMet-Leu-Phe stimulation in human neutrophils is metabolized largely by the kinase and not the lipase, which implies that diacylglycerol is not the major source of arachidonate during signal-transduction.
一种特定的二酰基甘油激酶抑制剂，浓度为10⁻⁵ M时，能持续增强用fMet-Leu-Phe、IgG、热聚集IgG和调理酵母聚糖刺激的人中性粒细胞产生超氧化物的能力。fMet-Leu-Phe的浓度-反应曲线向左移位，并且R59022也使超氧化物的最大释放量持续增加，而10⁻⁵ M的二酰基甘油脂肪酶抑制剂RHC80267则没有显著影响。这些结果表明，人中性粒细胞在fMet-Leu-Phe刺激后形成的二酰基甘油主要由激酶而非脂肪酶代谢，这意味着二酰基甘油不是信号转导过程中花生四烯酸的主要来源。
