Di Fronzo G, Cappelletti V, Miodini P, Bertario L, Ravasi G
Cancer Detect Prev. 1987;10(5-6):327-33.
The presence of cellular retinoic acid binding protein (cRABP) was analyzed in 13 consecutive patients with large bowel cancer (one right colon and 12 rectum-sigmoid, classified as three Duke B, eight C, and two D). Specimens of neoplastic tissue and of adjacent mucosa were obtained at surgery, and cRABP was determined by an assay based on incubation of partially purified cytosol with labeled retinoic acid and ultracentrifugation in sucrose density gradients. Sixty-one percent of tumors contained detectable levels of cRABP, whereas 58.3% of normal mucosal specimens were positive for cRABP. Among the positive tumors 62.5% contained cRABP also in the corresponding mucosa; in the group of cRABP-negative tumors, 40% showed cRABP in the adjacent mucosa. No correlation could be established with the grading or the stage of the tumors; however, interestingly, 100% (three cases) of gelatinous carcinomas were cRABP positive. Since cRABP seems to be a character of neoplastic cells contrary to normal ones, it would be interesting to investigate the conditions that influence the presence of this protein in normal appearing mucosa adjacent to carcinoma.
对13例连续性大肠癌患者（1例右半结肠癌，12例直肠乙状结肠癌，分为3例杜克B期、8例C期和2例D期）的细胞视黄酸结合蛋白（cRABP）进行了分析。手术时获取肿瘤组织和相邻黏膜的标本，通过将部分纯化的胞质溶胶与标记视黄酸孵育并在蔗糖密度梯度中进行超速离心的检测方法来测定cRABP。61%的肿瘤含有可检测水平的cRABP，而58.3%的正常黏膜标本cRABP呈阳性。在阳性肿瘤中，62.5%在相应黏膜中也含有cRABP；在cRABP阴性肿瘤组中，40%在相邻黏膜中显示有cRABP。无法与肿瘤的分级或分期建立相关性；然而，有趣的是，黏液腺癌100%（3例）cRABP呈阳性。由于与正常细胞相反，cRABP似乎是肿瘤细胞的一个特征，因此研究影响癌旁看似正常黏膜中该蛋白存在的条件将很有意思。
