School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.
College of Chemistry and Material Sciences, South-Central University for Nationalities, Wuhan, China.
J Cell Mol Med. 2018 Dec;22(12):6026-6038. doi: 10.1111/jcmm.13876. Epub 2018 Oct 16.
Stauntonia chinensis DC. has been utilised as a traditional herbal medicine for its anti-hyperglycemic characteristic, which has been associated with triterpene saponins. The goal of the current evaluation was to examine hypoglycemic activity and affiliated mechanism of total saponins from S. chinensis. The chemical composition was analysed by HPLC-ESI-MS/MS. The fasting blood glucose, oral glucose tolerance test, insulin tolerance test, insulin and glycogen levels of type 2 diabetic db/db mice administered total saponins were quantified to determine the hypoglycemic effects. The serum lipid profiles were assessed to determine the hypolipidemic effects. Western blotting was used to quantify the protein levels of insulin receptor substrates (IRS)-1/PI3K/AKT, AMPK/ACC and GLUT4. Twenty triterpene saponins were identified from the total saponins, which exhibited hypoglycemic activities and modulated hyperlipidemia that was associated with type 2 diabetes. The hypoglycemic effects were partly due to the activation of GLUT4, which is regulated by IRS-1/PI3K/AKT. The activation of the AMPK/ACC signalling pathway may be responsible for the hypolipidemic activity. This study revealed that total saponins from S. chinensis have significant hypoglycemic and hypolipidemic activity in diabetic db/db mice, indicating that these may be utilised in the development of saponins based on S. chinensis for the treatment of type 2 diabetes.
华中五味子 DC. 被用作一种传统的草药，具有降血糖的特性，这与三萜皂苷有关。本评价的目的是研究华中五味子总皂苷的降血糖活性及相关机制。采用 HPLC-ESI-MS/MS 分析化学成分。测定给予总皂苷的 2 型糖尿病 db/db 小鼠的空腹血糖、口服糖耐量试验、胰岛素耐量试验、胰岛素和糖原水平，以确定降血糖作用。测定血清脂质谱，以确定降血脂作用。采用 Western blot 测定胰岛素受体底物 (IRS)-1/PI3K/AKT、AMPK/ACC 和 GLUT4 的蛋白水平。从总皂苷中鉴定出 20 种三萜皂苷，它们具有降血糖活性，并调节与 2 型糖尿病相关的高脂血症。降血糖作用部分归因于 GLUT4 的激活，GLUT4 受 IRS-1/PI3K/AKT 调节。AMPK/ACC 信号通路的激活可能是降血脂活性的原因。本研究表明，华中五味子总皂苷对糖尿病 db/db 小鼠具有显著的降血糖和降血脂活性，表明这些总皂苷可能被用于开发基于华中五味子的治疗 2 型糖尿病的皂苷药物。
