Kristono Gisela A, Shorter Caroline, Pierse Nevil, Crane Julian, Siebers Robert
a Wellington Asthma Research Group, Department of Medicine , University of Otago , Wellington , New Zealand.
b Department of Public Health , University of Otago , Wellington , New Zealand.
J Occup Environ Hyg. 2019 Jan;16(1):89-96. doi: 10.1080/15459624.2018.1536827. Epub 2019 Jan 28.
Environmental exposure to endotoxin, Fel d I (cat) allergen and Der p I (house dust mite) allergen have been associated with asthma symptoms and have been measured in the environment using various sampling methods, including the electrostatic dust collector. The objectives of this study were to investigate whether levels of endotoxin and allergens were detectable in electrostatic dust collectors and to examine the correlation of allergen and endotoxin levels between electrostatic dust collectors and vacuum sampling methods (floor dust and mattress dust). Electrostatic cloths, bedroom floor dust and mattress dust samples from a subset of 60 homes were randomly selected from the Health of Occupants of Mouldy Homes study for allergen and endotoxin analysis. Fel d I and Der p I allergens were analyzed by double monoclonal antibody ELISA and endotoxin by the kinetic Limulus amoebocyte lysate assay. An enhanced ELISA method was used to analyze Der p I in the electrostatic cloths. Endotoxin was detected in all samples, however Fel d I and Der p I were not detected in all electrostatic dust collector samples (detection in 53% and 15% of cloths respectively). No correlations were found between cloth and dust samples for endotoxin or Der p I, but moderate-to-strong correlations were found between all three sampling methods for Fel d I (r = 0.612-0.715, p < 0.001). Poor correlation was found between floor dust and mattress dust samples for Der p I (r = 0.256, p = 0.048). Electrostatic dust collectors may provide a way to measure airborne dust and allergen. Given the moderate-to-low correlations with vacuum dust sampling, this may present a unique measurement system which, when collected alongside traditional vacuum dust sampling, could provide additional exposure measures. Further studies are required to correlate endotoxin and allergen levels measured by electrostatic dust collector with air sampling and to explore the relationships between these bioaerosols, environmental factors and asthma.
环境中接触内毒素、猫过敏原Fel d I和屋尘螨过敏原Der p I与哮喘症状有关，并且已使用包括静电集尘器在内的各种采样方法在环境中进行了测量。本研究的目的是调查静电集尘器中是否可检测到内毒素和过敏原水平，并检验静电集尘器与真空采样方法（地板灰尘和床垫灰尘）之间过敏原和内毒素水平的相关性。从“发霉房屋居住者健康”研究中随机选取60户家庭中的一部分的静电布、卧室地板灰尘和床垫灰尘样本，进行过敏原和内毒素分析。通过双单克隆抗体ELISA分析Fel d I和Der p I过敏原，通过动态鲎试剂法分析内毒素。采用改进的ELISA方法分析静电布中的Der p I。所有样本中均检测到内毒素，然而并非所有静电集尘器样本中都检测到Fel d I和Der p I（分别在53%和15%的布中检测到）。对于内毒素或Der p I，在布样本和灰尘样本之间未发现相关性，但对于Fel d I，在所有三种采样方法之间发现了中度至强的相关性（r = 0.612 - 0.715，p < 0.001）。对于Der p I，在地板灰尘和床垫灰尘样本之间发现相关性较差（r = 0.256，p = 0.048）。静电集尘器可能提供一种测量空气中灰尘和过敏原的方法。鉴于与真空灰尘采样的相关性为中度至低度，这可能是一种独特的测量系统，当与传统真空灰尘采样一起收集时，可以提供额外的暴露测量。需要进一步的研究来关联静电集尘器测量的内毒素和过敏原水平与空气采样，并探索这些生物气溶胶、环境因素与哮喘之间的关系。
