Department of Biological Science, University of Tulsa, 74104, Tulsa, OK, USA.
Department of Biological Sciences, LeTourneau University, 75602, Longview, TX, USA.
Curr Allergy Asthma Rep. 2023 May;23(5):223-236. doi: 10.1007/s11882-023-01073-2. Epub 2023 Mar 18.
To review current air sampling instruments and analysis methods and to describe new approaches being developed.
Spore trap sampling with analysis by microscopy remains the most widely used methods for aeroallergen determination even though there are often long delays from sample acquisition to data availability, as well as a need for specially-trained staff for sample analysis. The use of immunoassays and molecular biology to analyze outdoor or indoor samples has expanded in recent years and has provided valuable data on allergen exposure. New automated sampling devices capture pollen, analyze, and identify pollen grains by light scattering, laser-induced fluorescence, microscopy, or holography using signal or image processing to classify the pollen in real time or near real time. Air sampling data from current methods provide valuable information on aeroallergen exposure. The automated devices in use and under development show great potential but are not ready to replace existing aeroallergen networks.
回顾当前的空气采样仪器和分析方法，并描述正在开发的新方法。
尽管从采集样本到获得数据通常存在很长的延迟，并且需要专门的人员进行样本分析，但孢子陷阱采样和显微镜分析仍然是最广泛用于确定气传过敏原的方法。近年来，免疫分析和分子生物学已被用于分析户外或室内样本，为过敏原暴露提供了有价值的数据。新型自动化采样设备通过光散射、激光诱导荧光、显微镜或全息术捕获花粉，并通过信号或图像处理进行分析和识别花粉粒，实时或接近实时地对花粉进行分类。当前方法的空气采样数据提供了有关气传过敏原暴露的有价值信息。正在使用和开发中的自动化设备具有很大的潜力，但还没有准备好替代现有的气传过敏原网络。
