Van Dyck E, Piot P
Eur J Clin Microbiol. 1987 Feb;6(1):40-3. doi: 10.1007/BF02097188.
Two hundred strains of Haemophilus ducreyi, isolated in different parts of the world, were investigated using the API-ZYM system, which included 95 different substrates. All strains produced aminopeptidase against beta-naphthylamide derivatives of L-lysine, glycine, L-arginine, L-alanine, D-L-methionine, glycyl-glycine, glycyl-L-alanine and L-leucine. All strains also produced alkaline and acid phosphatase and phosphohydrolase. Nearly all strains showed esterase activity against butyrate, valerate, caproate and caprylate, but the reactions were very weak. No glycosidase activity could be detected. Of the 47 aminopeptidase tests showing variable reactions, only the results for S-benzyl-L-cystine, L-ornithine, L-alanyl-L-phenyl-alanyl-L-proline, L-hystidyl-L-leucyl-L-histidine and L-histidyl-L-serine arylamidase obtained on strains from Asia, Africa and Europe were significantly different (p less than 0.05). On the basis of test results for L-ornithine arylamidase and L-alanyl-L-phenyl-alanyl-L-proline arylamidase, the distribution of three biovars found among the isolates of the different continents was significantly different (p less than 0.0001), whereas African strains isolated in Kenya and South Africa yielded the same enzymatic pattern. Thus, these enzymes may constitute a marker system for the epidemiological study of Haemophilus ducreyi.
使用包含95种不同底物的API-ZYM系统，对从世界不同地区分离出的200株杜克雷嗜血杆菌进行了研究。所有菌株均产生针对L-赖氨酸、甘氨酸、L-精氨酸、L-丙氨酸、D-L-蛋氨酸、甘氨酰甘氨酸、甘氨酰-L-丙氨酸和L-亮氨酸的β-萘酰胺衍生物的氨肽酶。所有菌株还产生碱性和酸性磷酸酶以及磷酸水解酶。几乎所有菌株都显示出针对丁酸盐、戊酸盐、己酸盐和辛酸盐的酯酶活性，但反应非常微弱。未检测到糖苷酶活性。在47项显示出不同反应的氨肽酶测试中，只有从亚洲、非洲和欧洲菌株获得的S-苄基-L-胱氨酸、L-鸟氨酸、L-丙氨酰-L-苯丙氨酰-L-脯氨酸、L-组氨酰-L-亮氨酰-L-组氨酸和L-组氨酰-L-丝氨酸芳基酰胺酶的结果存在显著差异（p小于0.05）。基于L-鸟氨酸芳基酰胺酶和L-丙氨酰-L-苯丙氨酰-L-脯氨酸芳基酰胺酶的测试结果，在不同大陆分离株中发现的三种生物变种的分布存在显著差异（p小于0.0001），而在肯尼亚和南非分离的非洲菌株产生相同的酶模式。因此，这些酶可能构成杜克雷嗜血杆菌流行病学研究的标记系统。
