Borisova E A, Chukhlovin A B, Seiliev A A, Zherbin E A, Zhivotovsky B D, Hanson K P
Int J Radiat Biol Relat Stud Phys Chem Med. 1987 Mar;51(3):421-8. doi: 10.1080/09553008714550911.
The relationship between nuclear chromatin degradation to polydeoxyribonucleotides (PDN) and other features of interphase death were studied using thymocytes of normal and X-irradiated rats. Fractionation of the thymic cells in Percoll gradients was performed in order to separate dead from intact cells. The degree of radiation-induced chromatin fragmentation, as assessed by electrophoresis, was similar for PDN from all Percoll bands. Following irradiation 87-98 per cent of 'heavy' thymocytes were pyknotic and almost devoid of receptors to autologous erythrocytes thus comprising a dead cell population. A direct relationship between PDN content and nuclear pyknosis was noted throughout the gradient. The loss of autologous rosette-forming ability was directly related to other indices of interphase death. The possibility of PDN originating from pyknosis-prone cells and the capacity of radiosensitive thymocytes to form autologous rosettes are discussed.
利用正常和经X射线照射的大鼠胸腺细胞，研究了核染色质降解为多脱氧核糖核苷酸（PDN）与间期死亡的其他特征之间的关系。通过在Percoll梯度中对胸腺细胞进行分级分离，以将死细胞与完整细胞分开。通过电泳评估，来自所有Percoll条带的PDN的辐射诱导染色质片段化程度相似。照射后，87%-98%的“重”胸腺细胞呈固缩状，几乎没有自体红细胞受体，因此构成一个死细胞群体。在整个梯度中都注意到PDN含量与核固缩之间存在直接关系。自体玫瑰花结形成能力的丧失与间期死亡的其他指标直接相关。文中讨论了PDN源自易发生固缩的细胞的可能性以及放射敏感胸腺细胞形成自体玫瑰花结的能力。
