Flomenberg P R, Chen M, Munk G, Horwitz M S
J Infect Dis. 1987 Jun;155(6):1127-34. doi: 10.1093/infdis/155.6.1127.
Adenovirus type 35 (Ad35) is an uncommon group B adenovirus that has been isolated nearly exclusively from immunosuppressed hosts. In our series of 46 patients with Ad35 isolates, 36 had AIDS or AIDS-related complex (ARC), seven patients were immunocompromised because of other diseases, and three patients were "normal." Neither patients with AIDS or ARC made specific antibody to Ad35, whereas antibody was present in one immunocompetent host and one renal transplant recipient. All isolates were identified as Ad35 by using genomic analysis with the restriction endonuclease SmaI, but many viruses exhibited other group B hemagglutinins, a property of the fiber protein. Further analysis of DNAs from 40 of these isolates, mapped by using the enzymes BamHI, HpaI, and PstI, revealed a total of 14 genomic variants from the Ad35 prototype. Seven of these genomic types, with either Ad7 or Ad11 hemagglutinin, had corresponding restriction site differences within the fiber gene, a result consistent with recombination events with other group B adenoviruses.
35型腺病毒（Ad35）是一种不常见的B组腺病毒，几乎仅从免疫抑制宿主中分离得到。在我们收集的46例分离出Ad35的患者中，36例患有艾滋病或艾滋病相关综合征（ARC），7例因其他疾病导致免疫功能低下，3例为“正常”患者。艾滋病或ARC患者均未产生针对Ad35的特异性抗体，而在1名免疫功能正常的宿主和1名肾移植受者体内检测到了抗体。通过使用限制性内切酶SmaI进行基因组分析，所有分离株均被鉴定为Ad35，但许多病毒表现出其他B组血凝素，这是纤维蛋白的一种特性。对其中40株分离株的DNA进一步分析，使用BamHI、HpaI和PstI酶进行图谱分析，结果显示与Ad35原型相比共有14种基因组变体。其中7种基因组类型带有Ad7或Ad11血凝素，在纤维基因内具有相应的限制性位点差异，这一结果与和其他B组腺病毒发生重组事件相符。
