Guo D F, Shibata R, Shinagawa M, Sato G, Aoki K, Sawada H
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido.
Microbiol Immunol. 1988;32(8):833-42. doi: 10.1111/j.1348-0421.1988.tb01444.x.
A total of 53 Ad3 isolates obtained from patients with acute conjunctivitis in Japan, Australia, and the Philippines during 1973 to 1986 were analyzed for their genome types with 4 restriction endonucleases, BamHI, BglII, HindIII, and SmaI. Two new genome types designated tentatively as Ad3f and Ad3g were identified by combination of BamHI and BglII in the isolates. The changes of restriction sites and sizes of restriction fragments in newly recognized Ad3f and Ad3g were located at the similar regions reported in other Ad3 genome types by O'Donnell et al (1986) on physical maps of the Ad3 prototype strain GB genome. In Japan, 46 Ad3 isolates obtained from 1983 to 1986 were either Ad3f or Ad3g. Yearly alternation of predominance of both genome types were observed in the northern part of Japan during the period. In Australia, two genome types Ad3p and Ad3f were found in 6 isolates, and the former was observed in the 3 isolates obtained before 1981 and the remaining 3 isolates were obtained after 1983. In the Philippines, the only isolate obtained in 1984 was Ad3p.
1973年至1986年间，从日本、澳大利亚和菲律宾急性结膜炎患者身上获取了53株腺病毒3型（Ad3）分离株，用4种限制性内切酶BamHI、BglII、HindIII和SmaI对其基因组类型进行了分析。通过BamHI和BglII组合在这些分离株中鉴定出两种新的基因组类型，暂定为Ad3f和Ad3g。新识别出的Ad3f和Ad3g中限制性位点和限制性片段大小的变化位于O'Donnell等人（1986年）在Ad3原型菌株GB基因组物理图谱上报道的其他Ad3基因组类型的相似区域。在日本，1983年至1986年间获得的46株Ad3分离株要么是Ad3f要么是Ad3g。在此期间，在日本北部观察到两种基因组类型的优势年度交替。在澳大利亚，在6株分离株中发现了两种基因组类型Ad3p和Ad3f，前者在1981年之前获得的3株分离株中观察到，其余3株分离株是在1983年之后获得的。在菲律宾，1984年获得的唯一一株分离株是Ad3p。
