Sasada M, Kubo A, Nishimura T, Kakita T, Moriguchi T, Yamamoto K, Uchino H
J Leukoc Biol. 1987 Apr;41(4):289-94. doi: 10.1002/jlb.41.4.289.
Freshly isolated human monocytes ingested and killed Candida albicans, and generated O2- H2O2 and .OH efficiently. When monocytes were cultured in vitro, these cells transformed into macrophages. Cultured monocytes retained their ingestive activity but lost their candidacidal activity almost completely after day 3. The release of O2- by monocytes decreased slightly with culture and that of .OH was markedly decreased on day 3 of culture. The activity of myeloperoxidase in the monocytes decreased with culture. These results suggested that the loss of candidacidal activity is due to the decrease of .OH generation and myeloperoxidase activity in cultured monocytes.
新鲜分离的人单核细胞能够摄取并杀死白色念珠菌，且能高效产生活性氧（O2-、H2O2和·OH）。当单核细胞在体外培养时，这些细胞会转变为巨噬细胞。培养的单核细胞保留了其摄取活性，但在培养3天后几乎完全丧失了杀念珠菌活性。单核细胞释放O2-的能力随培养时间略有下降，而·OH的释放在培养第3天时显著降低。单核细胞中的髓过氧化物酶活性随培养而降低。这些结果表明，杀念珠菌活性的丧失是由于培养的单核细胞中·OH生成减少和髓过氧化物酶活性降低所致。
